- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Human
- Target protein:CHAC1
- Description:Human CHAC1 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Human CHAC1 ELISA Kit exhibits high specificity and excellent specificity for the detection of human CHAC1. No significant cross-reactivity or interference between CHAC1 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:15,625 - 1000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:9,375 pg/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Human CHAC1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human CHAC1 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human CHAC1 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human CHAC1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for CHAC1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the CHAC1 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-CHAC1 Antibody, which binds the captured CHAC1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of CHAC1 captured in each well. The concentration of CHAC1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.