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Dispase (1 U/ml)
  STMC07923
 :  STEMCELL Technologies
 :  
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Dispase (1 U/ml)
  STMC07923
 :  STEMCELL Technologies
 :  

 

  • Pk:
    100 mL
  • Species:
    Non-human primate
    Human
    Rat
    Mouse
  • Description:
    Dispase (1 U/ml)

 

 

1 U/ml dispase in DMEM/F-12.

  • Achieve gentle dissociation in a wide variety of tissues
  • Optimised for enzymatic passaging of human ES and iPS cells

Use Dispase (1 U/ml) for gentle dissociation of a wide variety of tissues. This proteolytic dissociation reagent has been optimised for use in enzymatic passaging of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells. Incubation of minced tissue with pre-warmed dispase and gentle agitation will liberate cells with minimal cell damage. Pre-warmed dispase can also be used to harvest cells from tissue culture plastic.

This product contains 1 U/ml Dispase II (neutral protease from Bacillus polymyxa) dissolved in DMEM/F-12. Unlike trypsin, dispase is not inhibited by serum. Dispase activity is inhibited by EDTA and EGTA. Dispase should be removed from cell suspensions by centrifuging the cells followed by washing the cells with buffer or culture medium.

 : Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier's restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the 'Product Restrictions').