Culture medium kit for growth, and establishment of human pancreatic organoids.

Reliably culture pancreatic duct organoids to study the function of the ductal epithelium, model pancreatic diseases such as cancer or cystic fibrosis, and perform targeted drug screens or toxicity assays. With the PancreaCult™ (Human) culture medium system, you have a complete and robust workflow to establish, expand, and maintain pancreatic duct organoids. Pancreatic organoids can be established from fresh or frozen dissociated tissue in PancreaCult™ Organoid Initiation Medium (OIM), optimised to provide higher rates of organoid formation from tissue. Organoids can then be cultured in PancreaCult™ Organoid Growth Medium (OGM) for long-term maintenance, passaging, serum-free drug screening, or cryopreservation for future experiments. The optional removal of EGF from the medium supports the depletion of normal cells in KRAS-activated cancer organoid cultures. Organoids grown using the PancreaCult™ (Human) medium system can be adapted to various culture protocols, including two-dimensional Transwell® monolayer cultures, dilute Corning® Matrigel® suspension cultures, and high-throughput assay-compatible culture formats. Learn how to set up human pancreatic organoids for drug testing in high-throughput formats using the latest protocol.