Maturation kit for generation of functional sensory neurons from human iPS cell-derived neuronal precursor cells.

Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. STEMdiff™ Sensory Neuron Maturation Kit is the final step in a workflow beginning from human pluripotent stem cells (hPSCs) and leading to a population of sensory neurons that can be used for drug discovery and pain research applications. After obtaining PSC-derived neural crest cells (NCCs) with STEMdiff™ neural crest differentiation kit, use the serum-free STEMdiff™ sensory neuron differentiation Kit to generate sensory neuron precursors. STEMdiff™ sensory neuron maturation kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature.