Specifications
- Pk:0,05 mg
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:N-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimise freeze-thaw cycles.
- Endotoxin content:<0,1 ng/µg (1 IEU/µg) as determined by LAL test
- Protein synonyms:DUSP3|Dual specificity protein phosphatase 3|Dual specificity protein phosphatase VHR|VHR|Vaccinia H1-related phosphatase
- Protein/peptide name:VHR
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:22,6 kDa
- Sequence:Ser2-Pro185
- Formulation:Supplied as a 0,2 µm filtered solution of PBS, pH 7,4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the Lyophilised protein in ddH₂O. Please aliquot the reconstituted solution to minimise freeze-thaw cycles.
- Tested applications:Bioassay
Specifications
About this item
Human DUSP3 belongs to the dual specificity protein phosphatase subfamily. DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. DUSPs are major modulators of critical signalling pathways that are dysregulated in various diseases. They negatively regulate members of the mitogen-activated protein kinase superfamily, which are associated with cellular proliferation and differentiation. DUSP3 is expressed in human tissues including breast and ovarian.DUSP3 shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Human DUSP3 specifically dephosphorylates and inactivates ERK1 and ERK2.
- Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays.