Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised ActiveMax® Human IL-7, Tag Free (Cat. No. ACRBIL7-H4219) at 2 μg/ml (100 μl/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ACRBIL7-H5258) with a linear range of 4-31 ng/ml (QC tested).; Measured by its binding ability in a functional ELISA. Biotinylated Human TSLP (R127A, R130A), His,Avitag (Cat. No. ACRBTSP-H82E0) immobilised at 2 μg/ml (100 μl/well) via precoated 5 μg/ml (100 μl/well) of Human TSLP R, Fc Tag (Cat. No. ACRBTSR-H525a), can bind can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ACRBIL7-H5258) with a linear range of 1 - 20 ng/ml (Routinely tested).; Captured Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. ACRBIL7-H5258) on CM5 chip via anti-mouse antibodies surface can bind ActiveMax® Human IL-7, Tag Free (Cat. No. ACRBIL7-H4219) with an affinity constant of 13,3 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
• Gene ID:
  P16871-1
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  IL7Ra|CD127
• Protein/peptide name:
  IL-7 R alpha
• Purity:
  >90%
• Molecular weight:
  52,0 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT