Specifications

• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C, avoid light
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation,; Measured by its binding ability in a functional ELISA, Immobilised Anti-Human MSLN MAb at 10 μg/ml (100 μl/well) can bind FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat, No, MSN-HF253) with a linear range of 0,078-0,625 μg/ml (Ex,488 nm/Em,535 nm) (QC tested),; 293 cells were transfected with anti-MSLN-scFv and RFP tag, 2e5 of the cells were stained with B, FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat, No, MSN-HF253, 1 μg/ml) and C, FITC-labeled protein control, A, Non-transfected 293 cells and C, FITC-labeled protein control were used as negative control, RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Mesothelin (296-580), Fc Tag (Cat, No, MSN-HF253) (QC tested).
• Gene ID:
  Q13421-3 (R309P)
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  MPF|MSLN|Mesothelin
• Protein/peptide name:
  Mesothelin
• Purity:
  >95%
• Molecular weight:
  58,6 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4, Normally trehalose is added as protectant before Lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT, Avoid light