Fructose assay kit, Supelco®
Product Details & Documents
This kit is for the quantitative, enzymatic determination of fructose in food and other materials.
Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalysed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.
Kit components
Fructose standard 500 mg
Glucose assay reagent 1 ml/vial
Phosphoglucose isomerase 1 ml/vial
Specifications
Specifications
- Pk:1 KIT
- Description:Fructose Assay Kit
- Pk Info:Sufficient for 20 assays
- Cat. No.:FA20-1KT
- M-Clarity quality level:MQ100
Specifications
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