Sensitive fluorogenic substrate for TACE (TNFalpha converting enzyme, ADAM17). Also cleaved by ADAM10 and likely other ADAMs and MMPs. The kcat/Km of TACE for this substrate is 7-fold higher than for the same peptide with the Mca-Dpa fluorophore-quencher pair. FAM fluorescence is quenched by the TAMRA group until cleavage separates them. Ex.: 485 nm, Em.: 535 nm, although the following Ex/Em can also be used: 490,494/515,520 nm. This substrate is useful for inhibitor screening, kinetic analysis, and cellular activity assay.