Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Species:E. coli
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:RNA pyrophosphohydrolase
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:20.8 kDa
- Sequence:Met1-Gly176
- Formulation:Supplied as a 0,2 µm filtered solution of 50 mM Tris, 500 mM NaCl, 10% glycerol, pH 8,0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Specifications
About this item
Messenger RNA (mRNA) degradation plays a key role in the control of gene expression in all organisms by limiting the number of times that each mRNA molecule can be used as a template for protein synthesis. RNA pyrophosphohydrolase, also called RppH, is a master regulator of 5'-dependent mRNA decay. It accelerates the degradation of transcripts by removing pyrophosphate from the 5'-end of triphosphorylated RNA, leading to a more labile monophosphorylated state that can stimulate subsequent ribonuclease cleavage. RppH preferentially hydrolyses diadenosine penta-phosphate with ATP as one of the reaction products, and can be able to hydrolyse diadenosine hexa- and tetra-phosphate. However, this protein has no activity on diadenosine tri-phosphate, ADP-ribose, NADH and UDP-glucose. In the meningitis causing strain E. coli K1, it has been shown to play a role in HBMEC (human brain microvascular endothelial cells) invasion in vitro.
This recombinant protein can be used for biological assays. For research use only.