- Pk:50 µG
- Enzyme type:Recombinant
- Species:Mouse
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Carboxypeptidase A4
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:46,7 kDa
- Sequence:Gly17-Tyr420
- Formulation:Supplied as a 0,2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, 10% Glycerol, pH 8,0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Biological assays
Carboxypeptidase A4 (CPA4) is a member of the peptidase M14 family. CPA4 is metalloprotease that could be involved in the histone hyperacetylation pathway. CPA4 binds one zinc ion per subunit and could catalyse to release of a C-terminal amino acid, with preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val.They have distinct expression patterns and different specificities for example, preferentially cleaving aromatic (carboxypeptidase As) or basic (carboxypeptidase Bs) residues. Several, such as carboxypeptidase Xs, have lost their catalytic activity. Carboxypeptidases play important roles in digestion of food, processing of bioactive peptides and blood coagulation.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.