Order Entry
Belgium
ContactUsLinkComponent
 
Human recombinant PPIase H (from E. coli)
  PRSI91-233
 :  
Human recombinant PPIase H (from E. coli)
  PRSI91-233
 :  

 

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Tag sequence:
    N-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    PPIase H
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    21.4 kD
  • Sequence:
    Met1-Met177
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

 

 

Peptidyl-Prolyl Cis-Trans Isomerase H (PPIH) belongs to the Cyclophilin-type PPIase family that accelerate the folding of proteins. PPIases can catalyze the cis-trans isomerisaion of Proline Imidic peptide bonds in oligopeptides. PPIH participates in pre-mRNA splicing. It is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. In addition, PPIH has PPIase activity and may play a role as a chaperone mediating the interactions between different proteins inside the spliceosome.

Fusion-Tag: N-6 His tag

This recombinant protein can be used for biological assays. For research use only.