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E. coli recombinant lon protease (from E. coli)
E. coli recombinant lon protease (from E. coli)
Catalog # PRSI91-099
Supplier:  ProSci Inc.
CAS Number:  
E. coli recombinant lon protease (from E. coli)
Catalog # PRSI91-099
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    E. coli
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Lon Protease
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    88.56 kD
  • Sequence:
    Asn2-Lys784
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 50 mM TrisHCl, 100 mM KCl, 10% Glycerol, pH 7.5 . Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

Specifications

About this item

Lon Protease, is a member of the Lon protease family. They are found in archaea, bacteria and eukaryotes. Lon protease is ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. It required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. It degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long and binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, and UmuD. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.