Specifications

• Source:
  E. coli
• Species:
  Sheep
• Storage conditions:
  Lyophilized Leptin Antagonist Triple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below −18 °C. Upon reconstitution at 0. 1 Lep-tA mutant mg/ml and up to 2mM and filter sterilization Leptin mutant can be stored at 4 °C or even room temperature for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of carrier protein (0. 1% HSA or BSA) is suggested. Please prevent freeze/thaw cycles.
• Biological activity:
  Pegylated triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has profound weight gain effect (as compared to the non-pegylated antagonist like in mouse leptin antagonists), resulting mainly from increased food intake.
• Protein synonyms:
  LEP
• Protein/peptide name:
  Leptin Triple Antagonist, pegylated
• Purity:
  Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
• Solubility:
  It is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Formulation:
  The protein was lyophilized from concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.
• Tested applications:
  SDS-PAGE