Specifications

• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  For long term, store lyophilized AdipoQ at −20 °C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 °C for limited period of time; it does not show any change after two weeks at 4 °C. The lyophilized protein remains stable for 24 months when stored at −20 °C.
• Biological activity:
  In vitro gluconeogenesis assay in primary hepatocytes was performed, showing the Adiponectin human derived from mammalian cells can inhibit glucose production. The ED50 was ~6 ug/ml.
• Protein synonyms:
  ACDC
• Protein/peptide name:
  Adiponectin glycosilated HEK
• Purity:
  Greater than 98% as determined by HPLC and SDS PAGE.
• Solubility:
  Add 0.2 uml of deionized water and let the lyophilized pellet dissolve completely.
• Sequence:
  ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK.
• Formulation:
  Sterile filtered and lyophilized from 0.5 mg/ml in PBS buffer.
• Tested applications:
  SDS-PAGE