Solid phase biotinylation kits, EZ-Link™, Pierce™

This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.

• Fast labeling and purification – the entire procedure takes only one hour (two hours for sulfhydryl labeling kits)
• Easy removal of spent and excess labeling reagent – no need for dialysis or gel filtration
• Solid phase method allows initially dilute antibodies to be recovered in a smaller volume after labeling
• Specific protocols for antibody ensure appropriate level of labeling (2 to 5 biotins per antibody molecule), minimising possibility of inactivation caused by overlabeling
• No-Weigh™ single-dose microtube packaging* ensures that the biotin reagent is fully active for eight separate experiments

*A single sealed microtube containing 2 mg of reagent is reconstituted for each biotinylation. The No-Weigh™ packaging allows access to fresh reagent on-demand for each solid phase biotinylation reaction.