Biotinylated recombinant Protein G can be used to probe and detect mouse and human antibodies, especially IgG isotypes, in western blotting, ELISA, IHC and other immunoassay protocols.

• Contains two Fc-binding domains per protein
• Does not bind human IgM, IgD or IgA
• Efficient than Protein A for mouse (IgG1), human (IgG3) and rat, goat and cow antibodies
• Less efficient than Protein A for guinea pig, pig, dog and cat