The Biosensis Mature BDNF Rapid™ enzyme-linked immunosorbent assay (ELISA) kit is a sandwich ELISA that allows the quantification of BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), cell lysates, human milk and brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity.

This Mature BDNF Rapid™ ELISA kit employs a recombinant human BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure BDNF in all these species and uses the same antibodies and antigen.

The purpose of this kit is the in vitro quantitative determination of BDNF in samples such as serum, plasma (EDTA and citrate), cell culture supernatants and brain extracts.

This kit consists of a pre-coated mouse monoclonal anti-BDNF capture antibody, a biotinylated anti-BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance.

Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately. This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1 and 5%, respectively (Polacchini et al., 2015).