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You searched for: Nucleic Acid Reagents

Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

eQo One-Step ToughMix®

eQo One-Step ToughMix®

Supplier: Quantabio

No compromises. Unparalleled performance meets sustainability.

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Taq DNA polymerase, 1000 U

Supplier: Genscript

Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.

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T4 DNA polymerase

T4 DNA polymerase

Supplier: Thermo Fisher Scientific

T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.

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Restriction enzymes, EcoRII, 10 U/µl, Fermentas

Restriction enzymes, EcoRII, 10 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

CCWGG sites and cuts best at 37 °C in O buffer.

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Pfu DNA polymerase

Supplier: AGILENT

High-quality polymerases for difficult to amplify DNA targets.

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TriLink® Adenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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TriLink® Cytidine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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Restriction enzymes, NotI, 10 U/µl, Fermentas

Restriction enzymes, NotI, 10 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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AMPIGENE® cDNA synthesis kit

AMPIGENE® cDNA synthesis kit

Supplier: ENZO LIFE SCIENCES

AMPIGENE® cDNA Synthesis Kit for efficient and sensitive cDNA synthesis, for downstream qPCR.

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TriLink® Uridine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.

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TriLink® 2'-Deoxyadenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.

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VWR®, Fast HiFi DNA polymerase, 2X master mix

VWR®, Fast HiFi DNA polymerase, 2X master mix

Supplier: VWR Chemicals

Fast HiFi DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60X Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. lt is recommended for applications, which require extremely high fidelity such as cloning / sub cloning, NGS applications and mutagenesis.

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Cyanine 5-UTP (enhanced)

Cyanine 5-UTP (enhanced)

Supplier: ENZO LIFE SCIENCES

Cyanine 5-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP.

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AMPIGENE® 1-Step RT-PCR kit

Supplier: ENZO LIFE SCIENCES

AMPIGENE® 1-Step RT-PCR Kit for optimised and efficient cDNA synthesis and PCR in a single tube.

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Restriction enzymes, XhoI, FastDigest™, Fermentas

Restriction enzymes, XhoI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

FastDigest™ XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest™ and FastDigest™ Green reaction buffers.

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Restriction enzymes, BamHI, HC, Fermentas

Restriction enzymes, BamHI, HC, Fermentas

Supplier: Thermo Fisher Scientific

GATCC sites and cuts best at 37 °C in its own unique buffer.

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repliQa HiFi ToughMix

repliQa HiFi ToughMix

Supplier: Quantabio

Superior speed and inhibitor tolerance.

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PCR decontamination kit

Supplier: ENZO LIFE SCIENCES

Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.

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Blur 8 BioProbe®

Supplier: ENZO LIFE SCIENCES

The Blur 8 BioProbe® labeled probe is prepared by nick translation of a clone of the human alu repeat sequence. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

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Restriction enzymes, PstI, FastDigest™, Fermentas

Restriction enzymes, PstI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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VWR®, TEMPase Hot Start DNA polymerase

VWR®, TEMPase Hot Start DNA polymerase

Supplier: VWR Chemicals

VWR® TEMPase Hot Start DNA Polymerases are highly stable polymerases, featuring higher specificity, superior sensitivity and greater yields compared to standard DNA polymerases. These features make them well suited for the detection of low abundance targets. Other uses include screening, amplification of GC-rich sequences, multiplex PCR, direct PCR and qPCR. A glycerol-free TEMPase Hot Start DNA Polymerase is also available for automation and freeze drying.

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TriLink® 2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxyguanosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddGTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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TriLink® 2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxythymidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddTTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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TriLink® 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)

Supplier: TriLink BioTechnologies

2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.

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Restriction enzymes, BsmBI, FastDigest™, fermentas

Restriction enzymes, BsmBI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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TriLink® N6-Methyladenosine-5'-Triphosphate

Supplier: TriLink BioTechnologies

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.).

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AccuStart™ master mixes, long range SuperMix

AccuStart™ master mixes, long range SuperMix

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

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Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas

Restriction enzymes, Eco32I (EcoRV) (HC), Fermentas

Supplier: Thermo Fisher Scientific

ATC sites and cuts best at 37 °C in R buffer Isoschizomers: EcoRV.

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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX

Supplier: ENZO LIFE SCIENCES

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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Restriction enzymes, Restriction enzyme, Eco147I (StuI), Fermentas

Restriction enzymes, Restriction enzyme, Eco147I (StuI), Fermentas

Supplier: Thermo Fisher Scientific

CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

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