Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
DNA Library Prep Kit, sparQ
Supplier: Quantabio
An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.
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TriLink® Guanosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a ribose, and a 5′ triphosphate group for synthesis of RNA molecules.
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TriLink® 2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxyadenosine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddATP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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Mitochondria/Cytosol Fractionation Kit, Abcam
Supplier: Abcam
Mitochondria/cytosol fractionation kit provides a unique formulation of reagents for the effective isolation of highly enriched mitochondrial fractions from the cytosolic fraction of mammalian cells, including both apoptotic and non-apoptotic cells. - Cited in over 110 publications - Isolates mitochondrial and cytosolic fractions for studying apoptotic pathways and signal transduction - Simple and easy procedure for fractionation - Suitable for detecting translocation of factors between fractions via Western blotting (WB), ELISA, or other assays
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Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®
Supplier: OMEGA BIO-TEK
By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.
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qPCR master mixes, Fast Plus EvaGreen®
Supplier: Biotium
A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.
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T7 RNA polymerase
Supplier: Thermo Fisher Scientific
T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.
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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX
Supplier: Enzo Life Sciences
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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Restriction enzymes, NcoI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Montage® SEQ96 Sequencing Reaction Cleanup Kits
Supplier: Merck
The Montage® SEQ96 sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.
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Restriction enzymes, NotI, 50 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
GGCCGC sites and cuts best at 37 °C in O buffer.
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TriLink® Engineered RNase Inhibitor
Supplier: TriLink BioTechnologies
Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.
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PerfeCTa® PreAmp 5X SuperMix
Supplier: Quantabio
PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.
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Viral RNA Xpress kit, Mag-Bind®
Supplier: OMEGA BIO-TEK
Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.
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Taq DNA polymerase, 1000 U
Supplier: Genscript
Taq DNA polymerase is a thermostable DNA polymerase isolated from an E. coli strain that carries the Taq DNA polymerase gene.
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Mag-Bind® Plant DNA DS 96 kit
Supplier: OMEGA BIO-TEK
The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.
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