You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Ethanol Quantitation Kit
Supplier: AAT Bioquest
The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.
Expand 1 Items
Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit
Supplier: AAT Bioquest
Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.
Expand 1 Items
Peroxide assay kit, PEROXsay™
Supplier: G-Biosciences
PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.
Expand 2 Items
Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*
Supplier: AAT Bioquest
Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.
Expand 1 Items
Amplite® Fluorimetric Aldehyde Quantitation Kit
Supplier: AAT Bioquest
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
Expand 1 Items
Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*
Supplier: AAT Bioquest
Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.
Expand 1 Items
Serine hydrolase probes, ActivX™ FP
Supplier: Thermo Fisher Scientific
The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.
Expand 3 Items
Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.
Expand 1 Items
Amplite® Colorimetric Beta-Lactamase Activity Assay Kit
Supplier: AAT Bioquest
β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.
Expand 1 Items
Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit
Supplier: AAT Bioquest
Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.
Expand 1 Items
Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.
Expand 1 Items
Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.
Expand 1 Items
Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.
Expand 1 Items
Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.
Expand 1 Items
Amplite® Colorimetric Enterokinase Activity Assay Kit
Supplier: AAT Bioquest
Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.
Expand 1 Items
Amplite® Colorimetric Pyruvate Assay Kit
Supplier: AAT Bioquest
Pyruvate is an important chemical compound in intracellular metabolic pathways.
Expand 1 Items
Amplite® Colorimetric Maleimide Quantitation Kit
Supplier: AAT Bioquest
Maleimides can be directly assayed spectrophotometrically at 302 nm.
Expand 1 Items
Amplite® Colorimetric L-Alanine Assay Kit
Supplier: AAT Bioquest
L-alanine (L-Ala) plays a crucial role as a building block of important proteins.
Expand 1 Items
Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
Expand 1 Items
Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
Expand 1 Items
Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.
Expand 1 Items
Amplite® Fluorimetric Asparaginase Activity Assay Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.
Expand 1 Items
Amplite® Colorimetric Total NADP and NADPH Assay Kit
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
Expand 2 Items
Renilla luciferase flash assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.
Expand 2 Items
Cypridina luciferase flash assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.
Expand 2 Items
Quantitative peroxide assay kit (lipid), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
Expand 1 Items
Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
Expand 1 Items
Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit
Supplier: AAT Bioquest
Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.
Expand 1 Items
Renilla luciferase glow assay kits, Pierce™
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.
Expand 2 Items
Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific
Supplier: Thermo Fisher Scientific
The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.