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275 results for Enzyme Assays

You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Ethanol Quantitation Kit

Amplite® Ethanol Quantitation Kit

Supplier: AAT Bioquest

The ability to rapidly perform quantitative measurements of ethanol is highly desirable in life science research, clinical evaluations, and food and drug industries.

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Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Amplite® Fluorimetric α-Ketoglutarate Quantitation Kit

Supplier: AAT Bioquest

Alpha-ketoglutarate (α-ketoglutarate) is a key molecule in the Krebs cycle determining the overall rate of the citric acid cycle of the organism.

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Peroxide assay kit, PEROXsay™

Peroxide assay kit, PEROXsay™

Supplier: G-Biosciences

PEROXsay™ is a colorimetric quantitative peroxide assay that measures the oxidation of ferrous (Fe²⁺+) ions to ferric (Fe³⁺) ions. Peroxides react with a sugar alcohol converting it to a peroxyl radical that subsequently starts the oxidation of ferrous ions to ferric ions. The acidic pH of the assay allows the ferric (Fe³⁺) ion to complex with the colorimetric reagent, resulting in a change in absorbance that is proportional to the peroxide concentration.

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Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Amplite® Fluorimetric Protein Quantitation Kit *Orange Fluorescence*

Supplier: AAT Bioquest

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

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Amplite® Fluorimetric Aldehyde Quantitation Kit

Amplite® Fluorimetric Aldehyde Quantitation Kit

Supplier: AAT Bioquest

The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.

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Amplite® Fluorimetric Calcium Quantitation Kit  *Red Fluorescence*

Amplite® Fluorimetric Calcium Quantitation Kit *Red Fluorescence*

Supplier: AAT Bioquest

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Colorimetric Beta-Lactamase Activity Assay Kit

Amplite® Colorimetric Beta-Lactamase Activity Assay Kit

Supplier: AAT Bioquest

β-lactamases are a large family of enzymes capable of hydrolysing β-lactams.

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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT Bioquest

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.

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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.

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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.

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Amplite® Colorimetric Enterokinase Activity Assay Kit

Amplite® Colorimetric Enterokinase Activity Assay Kit

Supplier: AAT Bioquest

Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.

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Amplite® Colorimetric Pyruvate Assay Kit

Amplite® Colorimetric Pyruvate Assay Kit

Supplier: AAT Bioquest

Pyruvate is an important chemical compound in intracellular metabolic pathways.

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Amplite® Colorimetric Maleimide Quantitation Kit

Amplite® Colorimetric Maleimide Quantitation Kit

Supplier: AAT Bioquest

Maleimides can be directly assayed spectrophotometrically at 302 nm.

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Amplite® Colorimetric L-Alanine Assay Kit

Amplite® Colorimetric L-Alanine Assay Kit

Supplier: AAT Bioquest

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*

Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Fluorimetric Asparaginase Activity Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.

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Amplite® Colorimetric Total NADP and NADPH Assay Kit

Amplite® Colorimetric Total NADP and NADPH Assay Kit

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Renilla luciferase flash assay kit, Pierce™, Thermo Scientific

Renilla luciferase flash assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Renilla Luciferase Flash Assay Kit provides researchers with a highly sensitive intracellular assay for transcriptional activity of regulatory elements in mammalian whole cell lysate.

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Cypridina luciferase flash assay kit, Pierce™

Cypridina luciferase flash assay kit, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

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Quantitative peroxide assay kit (lipid), Pierce™

Quantitative peroxide assay kit (lipid), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

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Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NAD/NADH Ratio Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Amplite® Colorimetric Glucose-6-Phosphate Dehydrogenase (G6PD) Assay Kit

Supplier: AAT Bioquest

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step in the pentose phosphate pathway.

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Renilla luciferase glow assay kits, Pierce™

Renilla luciferase glow assay kits, Pierce™

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce renilla Luciferase glow assay kit provides an extremely bright and stable bioluminescence signal (half-life approx. 3 hours), especially in the presence of green renilla luciferase reporter.

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Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Renilla-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Renilla-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

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