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275 results for Enzyme Assays

 

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Quantitative peroxide assay kit (aqueous), Pierce™

Quantitative peroxide assay kit (aqueous), Pierce™

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

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Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Amplite® Colorimetric Hypochlorite (Hypochlorous Acid) Assay Kit

Supplier: AAT BIOQUEST

Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.

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Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Pierce™ Cypridina-Firefly Luciferase Dual Assay Kit

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Cypridina-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Cypridina and Red Firefly luciferase activity in mammalian whole cell lysates.

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Phosphatase assay kit

Phosphatase assay kit

Supplier: G-Biosciences

The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

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Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules.

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Amplite® Colorimetric Aldehyde Quantitation Kit

Amplite® Colorimetric Aldehyde Quantitation Kit

Supplier: AAT BIOQUEST

Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance.

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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit for Nanoparticles

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Beadlite™ Rapid Colorimetric Maleimide Quantitation Kit is an essential tool for researchers involved in bioconjugation research, specifically designed for the accurate quantification of maleimide functional groups on solid surfaces such as magnetic beads, polystyrene beads, or other microsphere nanoparticles.

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Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT BIOQUEST

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Alkaline Phosphatase Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric Glycerol Assay Kit

Amplite® Fluorimetric Glycerol Assay Kit

Supplier: AAT BIOQUEST

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT BIOQUEST

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Renilla Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Gaussia Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.

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Amplite® Cholesterol Quantitation Kit

Amplite® Cholesterol Quantitation Kit

Supplier: AAT BIOQUEST

Cholesterol is required to build and maintain cell membranes.

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Amplite® Colorimetric NADPH Assay Kit

Amplite® Colorimetric NADPH Assay Kit

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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MMP Green™ Substrate

MMP Green™ Substrate

Supplier: AAT BIOQUEST

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit

Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit

Supplier: AAT BIOQUEST

Protein thiols are very important to protein structure, protein function and biological system redox environment.

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Amplite® Colorimetric Hexokinase Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.

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Amplite® Colorimetric Hydrogen Peroxide Assay Kit

Amplite® Colorimetric Hydrogen Peroxide Assay Kit

Supplier: AAT BIOQUEST

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Total NADP and NADPH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Total NADP and NADPH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.

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SAM Methyltransferase assay, SAM510™

SAM Methyltransferase assay, SAM510™

Supplier: G-Biosciences

SAM510™ assay is a continuous enzyme coupled assay that can continuously monitor SAM-dependent methyltransferases without the use of radioactive labels or endpoint measurements.

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Fluorescent β-Galactosidase assay kit

Fluorescent β-Galactosidase assay kit

Supplier: G-Biosciences

The Fluorescent β-Galactosidase assay is used for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. The Fluorescent β-Galactosidase assay (MUG) provides a 96-well assay format for galactosidase activity that is suitable for high throughput applications. The production of the fluorphore is monitored at an emission/excitation wavelength of 365/460 nm.

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Kinase enrichment kits, Pierce™

Kinase enrichment kits, Pierce™

Supplier: Thermo Fisher Scientific

The Pierce™ Kinase Enrichment Kits utilise an ActivX™ ATP or ADP probe to covalently label the active site of ATPases, including chaperones and metabolic enzymes, to enable their selective enrichment using a desthiobiotin tag.

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Protease Screening Kit

Supplier: G-Biosciences

The Protease Screening Kit provides a simple and quick method for testing samples for proteolysis. Simply incubate sample in the reagent provided and obtain results.

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Active GTPase pull-down and detection kits

Active GTPase pull-down and detection kits

Supplier: Thermo Fisher Scientific

The Active GTPase Pull-Down and Detection Kits allows the monitoring of changes in GTPase activity levels by enriching and detecting the active (GTP-bound) state of a particular GTPase.

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Fluorescent β-Galactosidase assay kit

Fluorescent β-Galactosidase assay kit

Supplier: G-Biosciences

β-Galactosidase assay is for determining the β-galactosidase activity in the lysates of cells transfected with a β-galactosidase expression construct. It uses chlorophenol red-β-D-galactopyranoside (CPRG), which is up to 10 times more sensitive than the classic O-nitrophenyl-β-D-galactopyranoside (ONPG) β-Galactosidase assays. This increased sensitivity makes it simpler to measure β-galactosidase activity in cells that are difficult to transfect or have low β-galactosidase activity expression. β-Galactosidase activity is measured using a spectrophotometer or a microplate reader to determine the amount of substrate converted at 570 to 595 nm.

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