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Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Amplite® Fluorimetric HDAC Activity Assay Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

Histone deacetylases (HDAC) are a class of enzymes that remove acetyl groups from a ε-N-acetyl lysine amino acid on a histone.

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Serine hydrolase probes, ActivX™ FP

Serine hydrolase probes, ActivX™ FP

Supplier: Thermo Fisher Scientific

The ActivX® FP serine hydrolase probes enable selective labeling and enrichment of active serine hydrolases. Fluorophosphonate (FP) probes specifically label serines of enzymatically active serine hydrolases. FP probes are also effective for screening small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins. Depending on the active-site probe tag group and the enzyme class to be profiled, active site-labeled enzymes are detected and quantified by Western blot, fluorescent gel imaging or mass spectrometry.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Glutamic Acid Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Glutamic acid is one of the 20 proteinogenic amino acids.

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Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*

Supplier: AAT BIOQUEST

Caspases play important roles in apoptosis and cell signaling.

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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.

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Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Amplite® Human Serum Albumin (HSA) Site II Binding Assay Kit

Supplier: AAT BIOQUEST

Human serum albumin (HSA) is one of the most important carriers for acidic drugs in human plasma and has been shown to bind a large number of different compounds in a reversible manner.

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Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Peroxidase (HRP) Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Peroxidase is a small molecule (MW ~40 KD) that can usually be conjugated to an antibody in a 4:1 ratio.

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Amplite® Colorimetric Antioxidant Activity Assay Kit

Amplite® Colorimetric Antioxidant Activity Assay Kit

Supplier: AAT BIOQUEST

Reactive oxygen species (ROS) are essential in cell signaling and maintaining physiological cell metabolism.

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Amplite® Fluorimetric Xanthine Assay Kit

Amplite® Fluorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Colorimetric Lipase Activity Assay Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® colorimetric lipase activity assay kit offers a straightforward and efficient protocol for quantifying lipase activity.

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Amplite® Fluorimetric cADP-Ribose Assay Kit

Amplite® Fluorimetric cADP-Ribose Assay Kit

Supplier: AAT BIOQUEST

This cADP-ribose assay can be performed in a convenient 96-well or 384-well microtiter plate.

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Amplite® Rapid Colorimetric PE-Maleimide Quantitation Kit

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. The Amplite® rapid colorimetric PE-maleimide quantitation kit facilitates the quantification of maleimide groups on maleimide-activated PE proteins using a proprietary sensor, Maleimide 680™, which has an absorption peak at ~680 nm.

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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT BIOQUEST

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.

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Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Amplite® Universal Fluorimetric Kinase Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

The Amplite® Universal Kinase Assay Kit is based on the monitoring of ADP formation, which is directly proportional to enzyme phoshphotransferase activity and is measured fluorimetricaly. This kit provides a fast, simple, and homogeneous assay for measure kinases activities. The characteristics of its high sensitivity (<0.2 uM ADP), broad ATP tolerance (1-300 uM), non-antibody based, non-radioactive and no-wash method to detect the amount of ADP produced as a result of enzyme activity make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation with no separation steps required.

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Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit

Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit

Supplier: AAT BIOQUEST

Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.

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Amplite® Fluorimetric Alanine Aminotransferase Assay Kit

Amplite® Fluorimetric Alanine Aminotransferase Assay Kit

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

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Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Amplite® Fluorimetric Maleimide Quantitation Kit *Green Fluorescence*

Supplier: AAT BIOQUEST

A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins or proteins to other biomolecules.

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Amplite® Colorimetric Aldehyde Quantitation Kit

Amplite® Colorimetric Aldehyde Quantitation Kit

Supplier: AAT BIOQUEST

Rapid and accurate measurement of aldehydes is important task for biological research, food industry, chemical research and environmental pollution surveillance.

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Amplite® Colorimetric Xanthine Assay Kit

Amplite® Colorimetric Xanthine Assay Kit

Supplier: AAT BIOQUEST

Xanthine is a purine base found in most human body tissues and fluids.

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Amplite® Fluorimetric D-Lactate Assay Kit

Amplite® Fluorimetric D-Lactate Assay Kit

Supplier: AAT BIOQUEST

Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.

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Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Amplite® Fluorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Colorimetric Glucose Quantitation Kit

Amplite® Colorimetric Glucose Quantitation Kit

Supplier: AAT BIOQUEST

Glucose, a monosaccharide, is the most important carbohydrate in biology.

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Amplite® Colorimetric Tyrosinase Assay Kit

Amplite® Colorimetric Tyrosinase Assay Kit

Supplier: AAT BIOQUEST

Tyrosinase is of great interest to drug discovery, life science research, food industry and cosmetics industry since it plays an important role in the biosynthetic pathway of melanin.

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Amplite® Colorimetric  Glycerol 3-Phosphate (G3P) Assay Kit

Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit

Supplier: AAT BIOQUEST

Glycerol 3-Phosphate (G3P) is an important intermediate in glycolysis metabolic pathway.

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Amplite® Colorimetric L-Aspartate (Aspartic Acid)  Assay Kit

Amplite® Colorimetric L-Aspartate (Aspartic Acid) Assay Kit

Supplier: AAT BIOQUEST

Aspartate (or Aspartic acid) is a negatively charged, polar amino acid.

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Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*

Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*

Supplier: AAT BIOQUEST

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Amplite® Luciferase Reporter Gene Assay Kit *Bright Glow*

Supplier: AAT BIOQUEST

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*

Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*

Supplier: AAT BIOQUEST

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.

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Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*

Supplier: AAT BIOQUEST

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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