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You searched for: Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

ReadiUse™ NADPH Regenerating Kit

ReadiUse™ NADPH Regenerating Kit

Supplier: AAT Bioquest

NADPH provides the reducing equivalents for biosynthetic reactions and for oxidation-reduction involved in protection against the toxicity of ROS (reactive oxygen species).

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Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Amplite® Fluorimetric D-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT Bioquest

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Glutathione Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The monitoring of reduced and oxidised glutathione (GSH) in biological samples is essential for evaluating the redox and detoxification status of cells and tissues in relation to the protective role of glutathione against oxidative and free-radical-mediated cell injury.

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Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Acetylcholinesterase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Acetylcholinesterase, also known as AChE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group.

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Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Supplier: AAT Bioquest

The traditional Bradford protein assay is widely used for quantifying protein concentrations.

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Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric NAD Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NADH) and its oxidized form (NAD) are essential cofactors for many enzyme reactions found in living cells.

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Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Renin Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Renin is an enzyme that participates in the body's renin-angiotensin system (RAS) that mediates extracellular volume and arterial vasoconstriction.

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Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Glutathione Peroxidase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Glutathione peroxidase is an enzyme family with peroxidase activity to protect the organism from oxidative damage.

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Amplite® Fluorimetric L-Alanine Assay Kit

Amplite® Fluorimetric L-Alanine Assay Kit

Supplier: AAT Bioquest

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

Amplite® Fluorimetric Beta-Galactosidase Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

E. coli beta-galactosidase is a 464 Kd tetramer.

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Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Amplite® Fluorimetric Neuraminidase Assay Kit *Blue Fluorescence*

Supplier: AAT Bioquest

Neuraminidases, also called sialidases, are glycoside hydrolase enzymes that catalyze the hydrolysis of terminal sialic acid residues and neuraminic acid.

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Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Lysyl Oxidase Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Lysyl oxidase (LOX) is an extracellular enzyme that catalyses formation of aldehydes from lysine residues in collagen and elastin precursors.

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Amplite® Colorimetric Enterokinase Activity Assay Kit

Amplite® Colorimetric Enterokinase Activity Assay Kit

Supplier: AAT Bioquest

Enterokinase (also called enteropeptidase) is a serine protease produced by cells in the duodenal wall and is a key enzyme in human and animal digestion system.

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Amplite® Colorimetric Pyruvate Assay Kit

Amplite® Colorimetric Pyruvate Assay Kit

Supplier: AAT Bioquest

Pyruvate is an important chemical compound in intracellular metabolic pathways.

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Amplite® Colorimetric Maleimide Quantitation Kit

Amplite® Colorimetric Maleimide Quantitation Kit

Supplier: AAT Bioquest

Maleimides can be directly assayed spectrophotometrically at 302 nm.

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Amplite® Colorimetric L-Alanine Assay Kit

Amplite® Colorimetric L-Alanine Assay Kit

Supplier: AAT Bioquest

L-alanine (L-Ala) plays a crucial role as a building block of important proteins.

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Amplite® Colorimetric Calcium Quantitation Kit  *Blue Color*

Amplite® Colorimetric Calcium Quantitation Kit *Blue Color*

Supplier: AAT Bioquest

Calcium is essential for all living organisms, particularly in cell physiology, where movement of the calcium ion Ca²⁺ into and out of the cytoplasm functions as a signal for many cellular processes.

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Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

Monitoring of various protease activities has become a routine task for many biological laboratories.

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Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Amplite® Fluorimetric Butyrylcholinesterase Activity Assay Kit

Supplier: AAT Bioquest

Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesized liver and present in blood.

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Beadlite™ Rapid Colorimetric Thiol Quantitation Kit for Nanoparticles

Supplier: AAT Bioquest

Designed for consistent and dependable results. Beadlite™ Rapid Colorimetric Thiol Quantitation kit provides a robust method to quantify thiol-functionalised nanoparticle with our proprietary Thiolite™ 490 that has its maximum absorbance at ~490 nm.

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Amplite® Colorimetric NADP/NADPH Ratio Assay Kit

Amplite® Colorimetric NADP/NADPH Ratio Assay Kit

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

Amplite® Universal Fluorimetric MMP Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Oxaloacetate Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.

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Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*

Amplite® Colorimetric Ammonia Quantitation Kit *Blue Color*

Supplier: AAT Bioquest

Ammonia is an important source of nitrogen for living systems.

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Amplite® Fluorimetric Neutrophil Elastase Activity Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® fluorimetric neutrophil elastase activity assay kit provides a robust and accurate method for quantifying neutrophil elastase (NE) activity.

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Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Total NAD and NADH Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*

Amplite® Fluorimetric Hydrogen Peroxide Assay Kit *Red Fluorescence*

Supplier: AAT Bioquest

Hydrogen peroxide (H₂O₂) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*

Amplite® MMP-3 Activity Assay Kit *Green Fluorescence*

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Fluorimetric Asparaginase Activity Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® Fluorimetric Asparaginase Activity Assay Kit provides a simple and straightforward procedure for measuring Asparaginase activity in a variety of biological samples.

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Amplite® Colorimetric Total NADP and NADPH Assay Kit

Amplite® Colorimetric Total NADP and NADPH Assay Kit

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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