Cellular Assays

Supplier: Merck

The Glucose (HK) Assay Kit is for the quantitative, enzymatic determination of glucose in food and other material.

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ LNP Apparent pK_a Assay Kit (TNS Method) provides a robust and easy-to-use platform for testing the apparent/global pK_a of formulated LNPs.

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ BODIPY SM-102 LNP kit (loadable) provides cargo-ready, empty, BODIPY-labelled SM-102 LNPs. LNPs are capable of rapid encapsulation of nucleic acids, without the need for incubation steps, enabling subsequent delivery to target cells or other downstream research applications. Also demonstrate very low toxicity compared to traditional lipid-based transfection methods.

Supplier: Cayman Chemical

Cayman’s LipidLaunch™ LNP-0315 uptake kit (Green Fluorescence) is intended to serve as a starting point for laboratories to explore the uptake of LNPs by their target cell type without the need for specialised equipment. Optimal preparation conditions for the encapsulation of nucleic acids with LNPs must be determined by the end user.

Supplier: Biotium

Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry or microscopy.

Supplier: Molecular Devices

The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.

Supplier: Molecular Devices

CatchPoint™ cAMP Fluorescent Assay Kit measures cAMP levels via a competitive immunoassay format. Cell signaling via G protein-coupled receptors (GPCRs) can be assessed by monitoring the downstream effectors calcium or Cyclic AMP (cAMP). Monitoring levels of cAMP, a second messenger produced in response to activation of adenylate cyclase, is one of the most common ways to screen for agonists and antagonists of GPCRs.

Supplier: Molecular Devices

CatchPoint™ cGMP Fluorescent Assay Kits measure cGMP levels via a competitive immunoassay format. The second messenger cyclic GMP (cGMP) modulates cellular processes, including smooth muscle relaxation, kidney function, and inflammation, by activating effectors such as protein kinases, cGMP-dependent phosphodiesterases, and cGMP-gated ion channels.

Supplier: Biotium

Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

Supplier: Biotium

CellBrite kits are designed to label cell cytoplasmic membranes with a fluorescent membrane dye pre-dissolved as an optimised staining solution. By combining CellBrite membrane stains, it is possible to label multiple cell populations with distinctive colours, a capability useful for the studies of cell-cell interactions.

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

Supplier: Biotium

NucView® 488 caspase-3 substrate is a novel cell membrane-permeable fluorogenic caspase substrate designed for detecting caspase-3 activity within live cells in real time.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: Biotium

ATP-Glo™ Bioluminometric Cell Viability Assay offers a highly sensitive assay for quantifying ATP. The homogeneous assay involves a single addition of ATP-Glo™ Detection Cocktail directly to cells in culture medium.

Supplier: G-Biosciences

Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.

Supplier: Molecular Devices

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.