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Tests cellulaires

Tests cellulaires

Les essais cellulaires ont toujours été un outil puissant dans le laboratoire de recherche. Ils sont extrêmement polyvalents et peuvent être conçus pour mesurer pratiquement toute fonction cellulaire ou biochimique. La détection sensible de sondes fluorescentes aide à évaluer la signalisation cellulaire et les voies de mort cellulaire, ce qui est utile pour la quantification rapide de la viabilité cellulaire. Les essais cellulaires peuvent être utilisés pour mesurer les effets de différents traitements sur la viabilité cellulaire et pour évaluer les effets toxiques médiés par divers mécanismes.

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Myeloperoxidase fluorometric detection kit

Supplier: Enzo Life Sciences

The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.

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Mitochondria/cytosol fractionation kit

Supplier: Enzo Life Sciences

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

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NF-κB (p65) transcription factor assay kit

NF-κB (p65) transcription factor assay kit

Supplier: Cayman Chemical

NF-κB (p65) Transcription Factor Assay Kit is a 96-well assay for measurement of NF-κB (p65).

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CF® Dye Dextran 10000 MW, anionic and fixable

CF® Dye Dextran 10000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

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ER-ID™ red assay kit (GFP-CERTIFIED®)

ER-ID™ red assay kit (GFP-CERTIFIED®)

Supplier: Enzo Life Sciences

ER-ID® Red assay kit (GFP-CERTIFIED®) contains an endoplasmic reticulum-selective dye suitable for live cell, or detergent-permeabilized aldehyde-fixed cell staining.

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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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Triglyceride colorimetric assay kit

Supplier: Cayman Chemical

Triglyceride Colorimetric Assay Kit is a simple, reproducible and sensitive tool for assaying triglycerides in plasma, serum, cell lysates and tissue homogenate samples.

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Cell viability assay kits, EarlyTox™ live/dead assay kits

Cell viability assay kits, EarlyTox™ live/dead assay kits

Supplier: Molecular Devices

EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.

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QBT™ Fatty acid uptake assay kit

QBT™ Fatty acid uptake assay kit

Supplier: Molecular Devices

QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.

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EZAssay™ TBARS Estimation Kit for Lipid Peroxidation

Supplier: HIMEDIA

Cellular assay, EZAssay™ TBARS estimation kit for lipid peroxidation

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Cell viability assay kits

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

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ROS-ID™ total ROS detection kit

ROS-ID™ total ROS detection kit

Supplier: Enzo Life Sciences

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy and/or flow cytometry.

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CF® Dye Dextran 3500 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.

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PPARδ transcription factor assay kit

PPARδ transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARδ DNA binding activity.

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TUNEL assay kits

TUNEL assay kits

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit

Supplier: Enzo Life Sciences

The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.

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