Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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VWR®, Rapid Transfer buffer 10X

Supplier: VWR Chemicals

Rapid transfer buffer is a simple, single component system for quick and efficient transfer of proteins from SDS-PAGE gels to membranes for Western blotting applications. Transfer is completed in 10 or 20 minutes using a standard semi-dry or wet transfer apparatus, respectively.

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VWR®, Buffers, ammonia- ammonium chloride and ammonium chloride-ammonium hydroxide USP Test solutions (TS)

Supplier: VWR Chemicals

Ready to use test solutions (TS).

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VWR®RapidBlock™, Blocking solution, 10x

Supplier: VWR Chemicals

RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.

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VWR® AVS TITRINORM, Cesium chloride/lanthanum chloride buffer solution acc. to schinkel for atomic absorption spectroscopy (aas)

Supplier: VWR Chemicals

This material is intended for use as an ionisation buffer for AAS

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VWR® AVS TITRINORM Buffer Solutions, 20 °C

Supplier: VWR Chemicals

Solutions prepared from AnalaR® NORMAPUR® grade analytical reagents.

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VWR® AVS TITRINORM Buffer Solutions, CRMs, at 25 °C

Supplier: VWR Chemicals

pH Buffer solution, Phosphate, pH value: 7,41, Reference temperature: 25 °C, VWR®, Colourless

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VWR® UltraPure, Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: VWR Chemicals

Cell culture buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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VWR® AVS TITRINORM pH Buffer Solutions in Sachets, 20 °C

Supplier: VWR Chemicals

For calibration of pH meters.

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VWR® Tris Buffer Solution, pH 7,5 (1 mol/L), UltraPure Grade

Supplier: VWR Chemicals

TRIS buffer solution pH 7,5 (1 mol/l), VWR®

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR® Buffer, HEPES Solution pH 7,3 (1 mol/L)

Supplier: VWR Chemicals

HEPES buffer solution pH 7.3 (1 mol/l), VWR®

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VWR®, Cell culture salt solutions, Dulbecco's Phosphate Buffered Saline (DPBS)

Supplier: VWR Chemicals

DPBS is commonly used salt solution in cell enumeration as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS is also used in FACS (Fluorescence-Activated Cell Sorting) process to wash and re-suspend cells during the dissociation process, as well as for many analytical methods or biochemical treatments.

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VWR® Phosphate Buffered Saline (PBS), Low Phosphate, 10X

Supplier: VWR Chemicals

Irradiated concentrate of PBS, suitable for use in washing cells after dilution.

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J.T.Baker® DILUT-IT™, Buffer Solutions, Colour Coded, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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J.T.Baker® DILUT-IT™, Buffers, Acetate, Concentrated Solutions

Supplier: Avantor

Specially developed for dissolution testing of pharmaceutical dosage forms and drug discovery, easy to use DILUT-IT™ concentrates can reduce overall cost of ownership in the laboratory. Just add purified water and you are ready for testing.