You searched for: Buffers

Supplier: G-Biosciences

Mammalian Cell PE LB™ has been developed for extraction of total biologically active, soluble proteins from mammalian cultured cells. The Mammalian Cell PE LB™ is based on organic buffering agents and utilises a mild non-ionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents, phosphatase and protease inhibitors may be added into Mammalian Cell PE LB™. Mammalian Cell PE LB™ has been tested on a wide variety of mammalian cells and can be used for both suspension and adherent cells.

Supplier: Corning

Sodium Acetate is a common molecular biology reagent. All production lots are tested to ensure that they are free of RNAse. It is often used in buffer systems to maintain the pH of a given solution.

Supplier: MP Biomedicals

EDTA disodium salt, (2%) solution, in phosphate buffered saline without calcium and magnesium. Used as a gentle non-enzymatic cell dissociation reagent.

Supplier: MP Biomedicals

A peroxidase protective buffer which stabilises HRP conjugates. It has 500 ml of PBS, pH 7,2, containing 0,5% BSA, antimicrobial preservatives and proprietary stabilising compounds.

Supplier: Thermo Fisher Scientific

The M-PER Mammalian Protein Extraction Reagent is designed to provide highly efficient total protein extraction from cultured mammalian cells.

Supplier: Thermo Fisher Scientific

This kit is ideal for segregation and enrichment of proteins from five different cellular compartments.

Supplier: Thermo Fisher Scientific

The NE-PER Nuclear and Cytoplasmic Extraction Kit provides for efficient cell lysis and extraction of separate cytoplasmic and nuclear protein fractions in less than two hours. The nuclear protein extraction method involves simple, stepwise lysis of cells and centrifugal isolation of nuclear and cytoplasmic protein fractions. A benchtop microcentrifuge, tubes and pipettors are the only tools required. The NE-PER Reagents efficiently solubilise and separate cytoplasmic and nuclear proteins into fractions with minimal cross-contamination or interference from genomic DNA and mRNA. The isolated proteins can be used to perform immunoassays as well as protein interaction studies such as mobility shift assays (EMSA), co-immunoprecipitation (Co-IP) and pull-down assays.

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Letheen Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

Supplier: Thermo Fisher Scientific

The I-PER Insect Cell Protein Extraction Reagent enables gentle and effective extraction of soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture. The baculovirus insect cell expression system is an efficient and popular system for production of recombinant (eukaryotic) proteins in cell culture. Proteins expressed in baculoviral systems can be used for structural analyses, biochemical assays and a variety of other applications. I-PER Reagent maintains functionality of extracted proteins and is directly compatible with downstream applications such as protein assays, Western blotting and 6xHis-tagged protein purification.

Supplier: Thermo Fisher Scientific

This reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilised proteins.

Supplier: Thermo Fisher Scientific

This kit is ideal for cell fractionation and enrichment of proteins from five different cellular compartments within two hours.

Supplier: G-Biosciences

Insect PE LB™ has been developed for extraction of total biologically active, soluble proteins from adherent or suspension cultured insect cells, including Sf9 and Sf21. Insect PE LB™ utilises a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Insect PE LB™ is fully compatible with downstream processes, such as electrophoresis and chromatography. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Insect PE LB™.

Supplier: WTW

These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 4,5, autoclaved

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, RNAse free, Liquid

Supplier: Thermo Fisher Scientific

Thermo Fisher Scientific

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+.  These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity.  The use of EDTA therefore allows for greater control over many experiments.

Supplier: Thermo Fisher Scientific

Potassium dihydrogen phosphate and di-Sodium hydrogen phosphate in aqueous solution, Phosphate, pH value: 7,00, ±0,02

Supplier: Honeywell Chemicals

Matrix Modifier, 2% Potassium (K) in 1% HCl 1%

Supplier: Corning

Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.

Supplier: Thermo Fisher Scientific

Sodium acetate, 3M aq, solution, pH 7,0, autoclaved

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

Supplier: CPACHEM

Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.

Supplier: MP Biomedicals

Phosphate buffered salts, Dulbecco's formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to to irrigate, wash, and dilute mammalian cells.

Supplier: KLINIPATH

Buffered EDTA for decalcifying fluids.

Supplier: WTW

Ion strength adjustment solutions have multiple tasks: TISAB for fluoride measurements does not only adjust the pH value, by also decomplexes the fluoride-containing complex compounds. Other ion strength adjustment solutions ensure an adapted pH value as well as the addition of a suitable conducting salt to correctly capture the concentration.

Supplier: Honeywell Chemicals

Borate, pH value: 10.00, 9.988 - 10.012, Reference temperature: 20 °C, Blue

Supplier: Merck Millipore (Novagen)

BugBuster® 10X is a concentrated formulation of the proprietary detergents employed in BugBuster® without the addition of salts or buffer components. Concentrated BugBuster® provides a flexible alternative to the ready-to-use standard 1X BugBuster®, allowing user-defined dilution and addition of buffer components. BugBuster® 10X has all of the bioprocessing benefits of standard BugBuster® plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.

Supplier: Merck Millipore (Novagen)

BugBuster® (primary amine-free) Protein Extraction Reagent is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilisation or cross-linking. The PIPPS buffer used in BugBuster® (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster®, but will not complex metal ions, also making it ideally suited for extraction of metal dependent proteins.