Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

GST buffer kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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Tris-Glycine (TG) buffer,, MP Biomedicals

Supplier: MP Biomedicals

Tris-glycine buffer is used for western blotting and gel electrophoresis.

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Buffer solutions

Supplier: SI Analytics

Buffer solutions intended for extremely precise calibration.

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His buffer kit

Supplier: Cytiva

His Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of histidine-tagged proteins according to recommended protocols. Moreover, a method for optimization of imidazole concentration in the binding buffer is described.

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Protein A/G HP SpinTrap™ Buffer Kit

Supplier: Cytiva

Protein A/G HP SpinTrap™ Buffer kit is optimised for standard affinity-based enrichment/immunoprecipitation according to the protocol provided with protein A HP SpinTrap™, protein G HP SpinTrap™, and Ab SpinTrap™ columns.

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Ab buffer kit

Supplier: Cytiva

Ab buffer kit contains prepared buffer concentrates for binding, washing, and elution of IgG according to recommended protocols.

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DALT Buffer kit for precast gels

Supplier: Cytiva

DALT Buffer kit is used together with DALT Gel 12,5, which comprises concentrated buffers for running the gel, gel buffer for inserting the gel into DALT precast gel cassette and sealing solution for application of the IPG strip. The buffer in the gel is part of a buffer system that gives longer shelf-life.

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pH buffers, ready to use

Supplier: WTW

Every bottle has its own lot reference with the expiration date.

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Buffer solutions as per DIN 19266-01: 2000

Supplier: WTW

These standard buffer solutions (work reference buffer solutions) are widely used in precision applications.

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Hank's balanced salt solution, modified, MP Biomedicals

Supplier: MP Biomedicals

Cell culture buffer, Hank's balanced salt solution, modified (1X)

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HEPES Buffer, 1 M Sterile, Cell Culture Grade, MP Biomedicals

Supplier: MP Biomedicals

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions.

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Low pH SDS Sample Buffer

Supplier: EKSIGENT

SCIEX Low pH SDS Sample Buffer, 140 mL (100 mM Tris-HCl, pH 6.8, 1% SDS) allows you to use SCIEX’s Gold Standard CE-SDS with a low pH SDS sample buffer. Use of this buffer can increase non-reduced purity by decreasing method induced fragmentation. Less variability and lower RSDs means less troubleshooting and faster method development.

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SSC 20X buffer, MP Biomedicals

Supplier: MP Biomedicals

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions:

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Buffer, PBS solution 10X concentrate (phosphate buffered saline), MP Biomedicals

Supplier: MP Biomedicals

Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)

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Phosphate Buffer Mixed pH 4,0

Supplier: CPACHEM

Shelf Life on Ship Date: 12 months - Store under normal laboratory conditions, at temperatures between 15 to 25 °C.

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IPG buffers

Supplier: Cytiva

IPG buffers are ampholyte-containing buffer concentrates specifically formulated for use with Immobiline™ DryStrip gels for isoelectric focusing, run as the first dimension of 2-D electrophoresis or as a separate application. Using Immobiline DryStrip gels together with matching IPG buffer gives high sample loading capacity, improves resolution, and increases the probability of detecting low abundance proteins.