Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Solubilisation buffer, 2D-Xtract™

Supplier: G-Biosciences

A protein solubilisation buffer for 2D analysis must solubilise proteins effectively, without disturbing the native charge of the proteins. Urea based solubilisation buffers solubilise proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results.

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IEF Anode buffer (50X)

Supplier: Thermo Fisher Scientific

Running Buffer, IEF Anode buffer (50X)

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PIPES lysis buffer with NP-40 (2X)

Supplier: Thermo Fisher Scientific

PIPES lysis buffer with NP-40 (2X), Thermo Fisher Scientific

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Phosphate-buffered saline (PBS, 5X), with EDTA

Supplier: Thermo Fisher Scientific

Phosphate-buffered saline (PBS) with EDTA, 5X, Liquid

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Glycine, 0.2M buffer soln., pH 3.0

Supplier: Thermo Fisher Scientific

Glycine buffer solution, 0,2 M, pH value: 3,0, Odourless, Liquid

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MES lysis buffer with Triton® X-100

Supplier: Thermo Fisher Scientific

MES lysis buffer with Triton® X-100, Thermo Fisher Scientific

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Loading buffer, SDS 4X, for SDS-PAGE analysis of proteins, Sigma-Aldrich®

Supplier: Merck Millipore (Novagen)

Loading Buffer, 4X SDS sample buffer

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NP-40 lysis buffer (2X)

Supplier: Thermo Fisher Scientific

NP-40 lysis buffer (2X), Thermo Fisher Scientific

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Tween 20 washing buffer, 1,0% in PBS (20X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 1,0% in PBS (20X)

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TBS with Tween® (TBST), 20X solution, Molecular Biology Grade, ultrapure

Supplier: Thermo Fisher Scientific

TBS is used in Western blotting protocols.

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Hybridisation buffer, SSPE 20X, AccuGENE™

Supplier: LONZA

AccuGENE™ electrophoresis buffers are ready-to-use solutions ideal for a wide range of molecular biology applications.

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EDTA, 0.5M pH 8.0, Corning®

Supplier: Corning

EDTA (ethylenediaminetetraacetic acid) is a polyamino carboxylic acid. It is a colorless, water-soluble solid and is useful as a chelating agent due to its ability to "sequester" metal ions such as Ca2+ and Fe3+. These cations are responsible for various undesirable effects in molecular biological and cell culture processes. After being bound by EDTA, these metal ions remain in the solution but exhibit diminished reactivity. The use of EDTA therefore allows for greater control over many experiments.

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10X TAE buffer, liquid, Corning®

Supplier: Corning

Tris-Acetate-EDTA (TAE) buffer is used in DNA agarose gel electrophoresis, both in the agarose gel itself and the running buffer

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10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

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GST buffer kit

Supplier: Cytiva

GST Buffer Kit contains carefully prepared buffer concentrates for binding, washing, and elution of GST-tagged proteins according to recommended protocols.

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Buffer Capsules

Supplier: Greisinger

Cost-effective alternative: Buffer capsules and working sets. With buffer capsules, you can produce a 100 ml buffer solution in the most cost-effective manner. A particular advantage of the capsules is that their storage life is several years longer than that of ready-to-use solutions.