Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Millipore®
Supplier: Merck Millipore (Calbiochem)
Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)
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Tris Hydrochloride
Supplier: Merck Millipore (Calbiochem)
Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).
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ADA, 0,2 M Buffer Solutions
Supplier: Thermo Fisher Scientific
N-(2-Acetimido)iminodiacetic acid monosodium salt (ADA) buffer solutions PH values 6,0 to 7,5.
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Buffers, imidazole, 0,5 M solutions
Supplier: Thermo Fisher Scientific
Imidazole buffer solution, 0,5 M, pH value: 6,5, Liquid
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Buffers, MOPS, 1,0 M solutions
Supplier: Thermo Fisher Scientific
MOPS buffer solution pH 5,5 (1 mol/l), pH value: 5,5, Colourless, Odourless, Liquid
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Glycine, 0.2M buffer soln., pH 3.5
Supplier: Thermo Fisher Scientific
Glycine buffer solution, 0,2 M, pH value: 3,5, Odourless, Liquid
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IEF Sample buffer (4X) pH 3 to 7 with lysine
Supplier: Thermo Fisher Scientific
Loading Buffer, IEF Sample Buffer (4X) with Lysine
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Buffers, BIS-TRIS propane, 0,2 M solutions
Supplier: Thermo Fisher Scientific
2,2'-(Propane-1,3-diyldiimino)bis[2-(hydroxy methyl)propane-1,3-diol]
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Ionisation buffer solutions (CRMs), for AAS and ICP, Fluka™
Supplier: Honeywell Chemicals
Matrix Modifier, 2% Cesium (Cs) in 1% HNO₃
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Buffers, MES, 0,5 M solutions
Supplier: Thermo Fisher Scientific
MES, buffer solution, 0,5 M, pH value: 8,0
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Maleate buffers, 0,2 M solutions, pH 5,0 - 7,0
Supplier: Thermo Fisher Scientific
Maleate buffer, 0,2 M, pH value: 5,5, Colourless, Liquid
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10% SDS (Sodium Dodecyl Sulfate), Corning®
Supplier: Corning
Sodium Dodecyl Sulfate (SDS) is an anionic detergent with applications in SDS-PAGE protein analysis and nucleic acid purification. It is a wetting agent that is effective in both acid and alkaline solutions, and is most often used as a protein and lipid solubilization reagent. SDS is also a powerful protein denaturant. This 10% solution is prepared in 18 megaohm water and is 0.2µm filtered.
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Lysis buffer, 0,5% Tween 20, pH 8,0, for isolation of His-tag modified proteins
Supplier: Thermo Fisher Scientific
Lysis buffer, 0.5% Tween 20, pH 8.0, for isolation of His-tag modified proteins, Thermo Fisher Scientific
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Buffer, TRIS, 1,0 M Solution, pH 9,0
Supplier: Thermo Fisher Scientific
TRIS buffer solution pH 9 (1 mol/L), Odourless, Liquid
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Tris-HCl, 1M solution, pH 8,0, Molecular Biology Grade, Ultrapure
Supplier: Thermo Fisher Scientific
1M solution of Tris, Ultrapure in high purity distilled H₂O, adjusted to pH 8,0.
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: LONZA
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals
#: 20060.320
Supplier: VWR Chemicals