Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Phosphate-buffered saline (PBS, 10X), pH 7,6

Supplier: Thermo Fisher Scientific

Cell culture buffer, PBS, 10 X

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Krebs-Ringer solution, bicarbonate-buffered

Supplier: Thermo Fisher Scientific

Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.

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MES-SDS running buffer (20X)

Supplier: Thermo Fisher Scientific

Running Buffer, MES-SDS running buffer (20X)

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TE buffer, pH 8,1, RNAse free

Supplier: Thermo Fisher Scientific

Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.

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Kinase buffer II (5X)

Supplier: Thermo Fisher Scientific

Kinase buffer II (5X)

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Sodium bicarbonate, Corning®

Supplier: Corning

Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment

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HEPES Buffer, ES Qualified, HyClone™ AdvanceSTEM™

Supplier: HyClone products (Cytiva)

HyClone AdvanceSTEM ES Qualified HEPES provides maintenance of physiological pH in cell culture despite changes in carbon dioxide.

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MOPS-EDTA-sodium acetate buffer (MESA)

Supplier: Thermo Fisher Scientific

MOPS-EDTA-sodium acetate buffer (MESA), Liquid

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Alkaline running buffer solution

Supplier: Thermo Fisher Scientific

Running Buffer, Alkaline running buffer solution

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TE buffer, (20X), pH 8,0, autoclaved

Supplier: Thermo Fisher Scientific

TE buffer concentrate, autoclaved, 20X, pH value: 8,0, Liquid

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TE buffer, pH 7,4, RNAse free

Supplier: Thermo Fisher Scientific

TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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TAPS, 0,2 M buffer soln., pH 8,5

Supplier: Thermo Fisher Scientific

TAPS buffer soln, 0,2 M, pH value: 8,5, Liquid

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.