Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

10X Tris buffered saline, liquid, Corning®

Supplier: Corning

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range.

Expand 1 Items

Buffer, PBS tablets, pH 7,4, Millipore®

Supplier: Merck Millipore (Oncogene)

Developed for use in immunoassay applications.

Expand 1 Items

Buffer, triethylammonium acetate, 1 M solution, pH 7,0, Calbiochem®, Millipore®

Supplier: Merck Millipore (Calbiochem‎)

This buffer solution is used for the purification of chemically synthesised oligonucleotides by HPLC.

Expand 1 Items

Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

Expand 1 Items

Buffers, MES, 1,0 M solutions

Supplier: Thermo Fisher Scientific

MES, buffer solution, 1,0 M, pH value: 7.5, Liquid

Expand 15 Items

Tween 20 washing buffer, 0,5% in PBS (10X)

Supplier: Thermo Fisher Scientific

Washing buffer, Tween 20 washing buffer, 0,5% in PBS (10X)

Expand 2 Items

G-Biosciences ACD (Acid Citrate Dextrose) Solutions for Anticoagulants

Supplier: G-Biosciences

ACD (Acid Citrate Dextrose) Solution A and ACD (Acid Citrate Dextrose) Solution B are anticoagulants for whole blood and also act as acidifying agents to platelet poor plasma.

Expand 4 Items

Nitrocellulose stripping buffer (10X)

Supplier: Thermo Fisher Scientific

Electrophoresis, Nitrocellulose Stripping Buffer, 10X

Expand 2 Items

Cesium chloride - lanthanum chloride buffer, for flame AAS, TraceSELECT™, Fluka™

Supplier: Honeywell Chemicals

TraceSELECT™, for flame atomic absorption spectrometry.

Expand 1 Items

MNE buffer, pH 6,5

Supplier: Thermo Fisher Scientific

MNE buffer, pH 6.5, Thermo Fisher Scientific

Expand 2 Items

TRIS-buffered saline (TBS, 10X) pH 7,4, for Western blot

Supplier: Thermo Fisher Scientific

Washing buffer, TRIS-buffered saline (TBS, 10X)

Expand 3 Items

TRIS-glycine-SDS running buffer (10X), pH 8,5

Supplier: Thermo Fisher Scientific

Running Buffer, TRIS-glycine-SDS running buffer (10X)

Expand 3 Items

HEPES-buffered saline, pH 7,0 (2X for transfection)

Supplier: Thermo Fisher Scientific

Cell culture buffer, HEPES-buffered Saline

Expand 2 Items

Phosphate, 0,5M buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Phosphate buffer soln, 0,5 M, pH value: 6,5, Liquid

Expand 2 Items

Protein extraction reagent, BugBuster® HT, Millipore®

Supplier: Merck Millipore (Novagen)

BugBuster® HT Protein Extraction Reagent combines BugBuster® Protein Extraction Reagent and Benzonase® Nuclease in one convenient reagent. BugBuster® HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high throughput protein purifications.