Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Alkaline running buffer solution

Supplier: Thermo Fisher Scientific

Running Buffer, Alkaline running buffer solution

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TE buffer, (20X), pH 8,0, autoclaved

Supplier: Thermo Fisher Scientific

TE buffer concentrate, autoclaved, 20X, pH value: 8,0, Liquid

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TE buffer, pH 7,4, RNAse free

Supplier: Thermo Fisher Scientific

TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.

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Buffer, sodium acetate, 1M aq, soln,, pH 4,5, RNAse free

Supplier: Thermo Fisher Scientific

Sodium acetate, 1M aq, solution, pH 4,5, RNAse free, Odourless, Liquid

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TAPS, 0,2 M buffer soln., pH 8,5

Supplier: Thermo Fisher Scientific

TAPS buffer soln, 0,2 M, pH value: 8,5, Liquid

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Buffer, SSC solution 20X concentrate (saline-sodium citrate), Corning®

Supplier: Corning

20X Saline-Sodium Citrate (SSC) buffer is used in both nucleic acid hybridization and blot transfer procedures

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Hybridisation buffers, formamide-free, HYB-LINK™

Supplier: G-Biosciences

Hyb-LINK™ is a high performance formamide-free hybridisation buffer solution for Northern, Southern and Dot Blots, which increases sensitivity of random-primer DNA probes.

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Sodium Cacodylate, 0,1 M Buffer solution, pH 6,5

Supplier: Thermo Fisher Scientific

Liquid.

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Buffer, TE solution 100x concentrate (TRIS-EDTA buffer), Millipore®

Supplier: Merck Millipore (Calbiochem‎)

Cloning Buffer, TE buffer solution 100x concentrate (TRIS-EDTA buffer)

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Tris Hydrochloride

Supplier: Merck Millipore (Calbiochem‎)

Use of tetraethylammonium (TEA) and Tris loading for blocking TRPM7 channels in intact cells.: This study highlights the application of TRIS Hydrochloride in blocking TRPM7 channels, which is significant for cell signaling and ion channel regulation in pharmacological research (Holderby & Kozak, 2024).