An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
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Fluorescent protease assay, Fluoro™
Supplier: G-Biosciences
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.
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Protein quantitation assay kits, CB-X™ Protein assay
Supplier: G-Biosciences
CB-X™ Protein Assay is a Coomassie dye based protein assay based on the Bradford Protein Assay. For protein samples in simple, uncomplicated aqueous buffers CB-X™ is a highly sensitive, single reagent assay that can be performed in 5 minutes. For complicated protein samples, CB-X™ Protein Assay is supplied with reagents to clean up the samples and remove all reagents and chemicals that interfere with accurate protein estimation in a single step. These reagents include detergents, chaotropes, reducing agents, alkylating agents, sugars, high salt concentrations, buffering agents and chelating agents. The clean up stage and subsequent protein assay is performed in a single tube to ensure no protein loss and to maintain the accuracy of the assay.
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FLUOR DE LYS® SIRT3 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT3 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Cell-based assay calcein AM
Supplier: Cayman Chemical
Cellular assay, Cell-based assay calcein AM
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ALDetect™ Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.
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PPARδ transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARδ DNA binding activity.
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Thermal shift protein stability kits, GloMelt™
Supplier: Biotium
Kit for fluorescence-based measurement of protein unfolding or thermal stability in thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.
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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Cellular senescence live cell analysis assay
Supplier: ENZO LIFE SCIENCES
Simple assay to quantify senescence-associated β-galactosidase activity, in live cells, using flow cytometry or fluorescent microscopy.
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ViaFluor® 488 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with green fluorescence.
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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit
Supplier: ENZO LIFE SCIENCES
The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
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PPARα transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of PPARα DNA binding activity.
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Calcium assay kit, FluoForte®
Supplier: ENZO LIFE SCIENCES
FluoForte® Calcium Assay Kits detect mobilisation of intracellular calcium utilising a fluorogenic calcium-binding dye optimised for superior cell-permeability and retention. The self-quenching dye undergoes an electronic change upon binding of calcium, resulting in a several order of magnitude greater fluorescence.
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Bicinchoninic acid (BCA) reducing agent compatible protein Assay
Supplier: G-Biosciences
The Bicinchoninic Acid (BCA) Protein Assay is a highly sensitive colorimetric assay that is compatible with detergent solubilised protein solutions, however is dramatically affected by the presence of reducing agents, including DTT, ß-mercaptoethanol and TCEP.
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Ubiquitinylation kit
Supplier: ENZO LIFE SCIENCES
Versatile tool for generation of ubiquitin-E2 thioesters.
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Granzyme B assay kit for drug discovery
Supplier: ENZO LIFE SCIENCES
The Granzyme B Assay Kit for Drug Discovery is a complete assay system designed to measure the protease activity of granzyme B. It contains a colorimetric substrate (IEPD-pNA) to measure this activity; cleavage of the p-nitroanilide (pNA) group from the colorimetric substrate increases absorption at 405 nm.
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Calcium assay kits, FLIPR® Calcium 6 and 6-QF assay kits
Supplier: Molecular Devices
The FLIPR® Calcium 6 and 6-QF Assay Kits provide a fast, simple, and reliable fluorescence based assay for detecting changes in intracellular calcium. The Calcium 6 Assay Kit significantly reduces fluorescence background with a one-step protocol. The Calcium 6-QF Assay Kit provides a quench-free option for sensitive targets or multiplexing applications.
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dsDNA quantitation kits for the Qubit® fluorometer, AccuGreen™, high-sensitivity and broad-range
Supplier: Biotium
AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.
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ApoSENSOR™ ADP/ATP ratio assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
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LSD1 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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p70 S6K activity kit
Supplier: ENZO LIFE SCIENCES
The p70 S6K activity kit is an efficient assay that utilizes little sample volume. This kit gives you the ability to an end-point or kinetic assay read-out in a convenient 96-well plate based assay and offers easy sample handling protocols. The assay also offers a high signal to background ratio.
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HIF-1α transcription factor assay Kit
Supplier: Cayman Chemical
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.
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Protein quantitation assay kits, CB™ Protein assay
Supplier: G-Biosciences
CB™ Protein Assay, a Coomassie dye based protein assay based on the Bradford Protein Assay, is suitable for the simple and rapid estimation of protein concentration. The binding of protein to the dye results in a colour change from brown to blue. The change in colour density is proportional to protein concentration. Protein estimation can be performed using as little as 0,5 µg protein.
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α-Amylase Inhibitor Screening Kit
Supplier: Abcam
Simple, 1-step assay for screening potential α-amylase inhibitors.
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DPPIV/CD26 assay kit for biological samples
Supplier: ENZO LIFE SCIENCES
The DPPIV/CD26 Assay kit for biological samples is a complete assay system designed to measure DPPIV activity in biological fluids such as plasma, serum, urine, and saliva. Uses for the kit include correlation of DPPIV activity with disease states or determination of the efficacy of DPPIV inhibitors administered in vivo.
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Fluorometric HTS assay kits, Caspase-3 and Caspase-8
Supplier: Biotium
Caspase-3 and Caspase-8 Fluorometric HTS Assay Kits provide a homogenous assay system for fast and highly sensitive detection of Caspase-3 or Caspase-8 activity by fluorescence in enzymatic reaction or mammalian cells.