You searched for: Assays
An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Reporter assay kits, SpectraMax® Glo Steady-Luc™
Supplier: Molecular Devices
The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.
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PROTEOSTAT® thermal shift stability assay kit
Supplier: ENZO LIFE SCIENCES
This kit has been designed for monitoring protein stability under systematic thermal stress conditions.
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Potassium assay kits, FLIPR®
Supplier: Molecular Devices
The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.
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Cholesterol cell-based detection assay kit
Supplier: Cayman Chemical
Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.
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Comet SCGE assay kit
Supplier: ENZO LIFE SCIENCES
COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.
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LDH Cytotoxicity WST Assay
Supplier: ENZO LIFE SCIENCES
Colorimetric kit for the determination of cytotoxicity through the measurement of lactate dehydrogenase activity.
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Steady-Luc™ Firefly HTS assay kit
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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FLUOR DE LYS® HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
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SREBP-1 transcription factor assay kit
Supplier: Cayman Chemical
A 96-well assay for measurement of SREBP-1.
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ROS Detection Cell-Based Assay Kit (DHE)
Supplier: Cayman Chemical
Measure superoxide and hydrogen peroxide levels in living cells.
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Firefly luciferase assay kit (Lyophilised)
Supplier: Biotium
This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.
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Caspase-2 fluorometric assay kit
Supplier: ENZO LIFE SCIENCES
Based on the detection of AFC (7-amino-4-trifluoromethyl coumarin) after cleavage from the labelled substrate VDVAD-AFC.
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FLUOR DE LYS® HDAC fluorometric cellular activity assay kit
Supplier: ENZO LIFE SCIENCES
The HDAC fluorometric cellular activity assay allows the determination of deacetylase activity within an undisturbed cellular environment and provides accurate activity information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.
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Superoxide dismutase assay kit
Supplier: Cayman Chemical
Superoxide Dismutase Assay (SOD) kit is a fast and reliable assay for the measurement of SOD activity from plasma, serum, tissue homogenates and cell lysates.
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Fluorescent protease assay, Fluoro™
Supplier: G-Biosciences
The Fluoro™ Protease Assay Kit is designed for the quantitative determination of proteases present in a protein sample. The assay uses fluorescein isothiocyanate (FITC)-labelled casein as a general protease substrate. The fluorescein label on the FITC-casein is highly quenched. When the proteases present in the sample of interest digest the FITC-casein substrate into smaller peptides, the quenching of the fluorescence label is relieved and the fluorescence of the substrate is increased. The fluorescence of the FITC-labeled peptide is measured with excitation at 485 nm and emission at 530 nm to determine protease activity. The kit detects picogram level of proteases present in the sample.
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LEADING LIGHT® Wnt Reporter Assay Starter Kit
Supplier: ENZO LIFE SCIENCES
The LEADING LIGHT® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format.
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Calcium assay kits, FLIPR® Calcium 5 assay kit
Supplier: Molecular Devices
The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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OXI-TEK TBARS assay kit
Supplier: ENZO LIFE SCIENCES
The sensitivity of measuring thiobarbituric acid reactive substances (TBARS) has made this assay the method of choice for screening and monitoring lipid peroxidation, which is a major indication of oxidative stress.
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p53 transcription factor assay kit
Supplier: Cayman Chemical
96-Well assay for measurement of p53.
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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells
Supplier: Biotium
The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.
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eFluxx-ID® multidrug resistance assay kit
Supplier: ENZO LIFE SCIENCES
The EFLUXX-ID® Multidrug Resistance Assay Kit allows for functional detection of all three clinically relevant ABC transporter proteins MDR1 (p-glycoprotein), MRP1/2, and BCRP. The assay uses a hydrophobic, non-fluorescent compound that readily penetrates the cell membrane, where it is hydrolyzed to a hydrophilic fluorescent dye by intracellular esterases.
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Reagents for Fluor-de-Lys®-Green HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
Cellular assay, Fluor-de-Lys®-green substrate, in 50 mM solution in DMSO
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Cell viability assay kits, EarlyTox™ Caspase-3/7 R110 assay kit
Supplier: Molecular Devices
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.
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Glycolysis Cell-Based Assay Kit
Supplier: Cayman Chemical
A colorimetric-based method for detecting L-lactate in culture medium.
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Thiol Detection Assay Kits
Supplier: Cayman Chemical
A simple, reproducible, and sensitive tool for assaying free thiol content in samples.
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ALDetect™ Lipid Peroxidation Assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to measure either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid). It is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole with MDA and 4-hydroxyalkenals to yield a stable chromophore with maximal absorbance at 586 nm.
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Protein assay kits, RED 660™
Supplier: G-Biosciences
RED 660™ Protein Assay is a single reagent colorimetric assay that outperforms many commercial colorimetric assays, including Bradford and improved Coomassie/Bradford assays. It offers greater linearity, colour stability, and compatibility with detergents, reducing agents and other interfering agents compared to the Coomassie assays. The single, ready to use reagent allows for rapid analysis of total protein concentration and generates highly reproducible results.
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Steady-Luc™ Firefly HTS assay kit (lyophilised)
Supplier: Biotium
Firefly luciferase is widely used as a transcriptional reporter gene for research and drug screening. The Steady-Luc™ HTS kit can be used with luminescence plate readers without sample injection systems or for high-throughput screening.
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Reporter gene assays, SpectraMax® DuoLuc™ reporter assay kit
Supplier: Molecular Devices
SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.