You searched for: 5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Supplier: Apollo Scientific

5(6)-Carboxyfluorescein N-hydroxysuccinimide ester (5/6-FAM SE)

Supplier: Biotium

5(6)-FAM SE (full name: 5-(and-6)-Carboxyfluorescein, succinimidyl ester mixed isomers) is an amine-reactive green fluorescent dye widely used for labeling proteins or other molecules that contain a primary or secondary aliphatic amine. The coupling reaction is usually carried out at pH 8-9.5. The amide linkage from the coupling reaction is much more stable than the thiourea linkage formed from the coupling of an amine and an isothiocyanate.

Supplier: AAT BIOQUEST

Carboxyfluorescein (commonly called FAM) and its amine-reactive succinimidyl esters are favored over FITC in bioconjugations.

Supplier: Thermo Fisher Scientific

NHS fluorescein [(5/6-carboxyfluorescein succinimidyl ester), mixed isomer] is an amine reactive derivative of fluorescein dye for antibodies and other probes in fluorescence microscopy, flow cytometry, and immunofluorescence based assays such as western blotting and ELISA.