378 Results for: "VARLP080520VARI00001"

Supplier: SIGMA ALDRICH MICROSCOPY

Phenol Red sodium salt is widely used as a pH indicator. At a pH of 6,4 and below, phenol red solution will have a yellow colour. At a pH of 8,2 and above, it will have a red colour. It is commonly used in culture media to detect neutral to acidic pH changes.

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Supplier: SIGMA ALDRICH MICROSCOPY

Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa's staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

Supplier: SIGMA ALDRICH MICROSCOPY

Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525 nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitiser.

Supplier: SIGMA ALDRICH MICROSCOPY

Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6,4 or below and a red color at a pH of 8,2 and above.

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O, also known as basic red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalised positive charge, an alternation of aromatic and quinonoid structures, and diazotisable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Supplier: SIGMA ALDRICH MICROSCOPY

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.

Supplier: SIGMA ALDRICH MICROSCOPY

Acid Fuchsin is mainly used as a diachrome and is part of the connective tissue stains. It also works as a pH indicator.

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers and matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial's reagent for the determination of RNA, DNA, and hexoses.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol Blue sodium salt has been used as a pH indicator in the yeast growth media (YPD, yeast extract peptone dextrose).

Supplier: SIGMA ALDRICH MICROSCOPY

Folin & Ciocalteu's phenol reagent is most commonly used in the Lowry method for determining protein concentration. It has also been used for the quantification of total phenolics. In this method, protein is pretreated with copper(II) in a modified biuret reagent (alkaline copper solution
stabilized with sodium potassium tartrate). Addition of the phenol reagent generates chromogens that give increasing absorbance between 550-750nm. Normally, absorbance at the peak (750 nm) or shoulder (660 nm) are used to quantitate protein concentrations between 1 - 100 mg/ml while absorbance at 550 nm is used to quantitate higher protein concentrations.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromothymol blue has been used for freshwater pH measurements over a broad range of buffer intensities.

Supplier: SIGMA ALDRICH MICROSCOPY

Patent Blue VF has been used in combination with 99mTc phytate to detect sentinel lymph node (SLN) in early stage cervical cancer patients. To detect heat shock protein (HSP) gp96 induced draining lymph nodes (LNs) in mice and to detect the effect of Asn-Gly-Arg (NGR)-tumor necrosis factor (TNF) on tumor perfusion in murine model. It is also used a diagnostic assay manufacturing, hematology, histology.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol green is a sulfonephthalein dye. It is mainly used for measuring serum albumin. In acidic conditions, bromocresol green interacts with albumin, thereby resulting in an increase in absorbance at 630 nm. The change in absorbance is linked with the concentration of albumin. Bromocresol Green is also a pH indicator, changing from yellow (pH 3.8) to blue color (5.4). It is widely used as a colloidal pigment.

Supplier: SIGMA ALDRICH MICROSCOPY

Azomethine-H is used as a color development agent. It is used for the determination of boron, mainly in soil samples. Azomethine-H monosodium salt hydrate has been used for the colorimetric determination of boron in soil and plants.

Supplier: SIGMA ALDRICH MICROSCOPY

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta. Auramine O is used for the staining of acid-fast organisms, coccidia. Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall. Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

Supplier: SIGMA ALDRICH MICROSCOPY

Trypan blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Supplier: SIGMA ALDRICH MICROSCOPY

Trypan Blue (C.l. 23850) is a large, tetrasulfonated anionic, hydrophilic dye. It is also known as diamine blue 3B. Trypan blue has been used as a dye in trypan blue exclusion assay/cell viability assay to detect dead cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine orange is also used to analyse autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

Supplier: SIGMA ALDRICH MICROSCOPY

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slightly negative charge and will migrate in the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The tracking dye typically migrates with the DNA molecules around 5 kb. Xylene cyanol may also be used in loading buffer that facilitates the observation of the sample during gel loading and electrophoresis.

Supplier: SIGMA ALDRICH MICROSCOPY

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.

Supplier: SIGMA ALDRICH MICROSCOPY

ANSA (8-Anilino-1-naphthalenesulfonic acid) is a hydrophobic polarity sensitive fluorescent dye useful as a site probe to detect conformational changes in cell and micelle membranes and molecules such as proteins. ANSA is also used as a fluorescent probe for the estimation of the critical micelle concentration (CMC) of surfactants.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromophenol blue (BroB), an arylmethane color marker dye has a molecular mass of 670 Da.

Supplier: SIGMA ALDRICH MICROSCOPY

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes colour from yellow to blue-green at pH 3,8 to 5,4. The monosodium salt of bromocresol green is highly ionisable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.