2371 Results for: "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine that promotes the survival and proliferation of pluripotent hematopoietic stem cells and lineage-committed progenitor cells and their differentiation into mature cells of most lineages, including basophils, neutrophils, eosinophils, macrophages, dendritic cells, erythrocytes, and megakaryocytes (Yang et al.; Dorssers et al.; Broughton et al.). IL-3 is produced by activated T cells and has a physiological role in inflammation and allergies by promoting the secretion of inflammatory mediators such as histamine, IL-4, and IL-6 by basophils and eosinophils (Broughton et al.). The IL-3 receptor consists of a unique alpha subunit (CD123) and a beta common subunit (βc or CD131) that is shared with the receptors for IL-5 and GM-CSF, and is the principal signal transduction subunit for these cytokines. IL-3 binding to the heterodimeric receptor activates JAK/STAT, MAPK, and PI3K signaling pathways (Woodcock et al.).

Supplier: STEMCELL Technologies

For culture and evaluation of mouse mammary epithelial cells.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection kit using particle release technology

Supplier: STEMCELL Technologies

Column-free immunomagnetic positive selection of human CD3+ cells for HLA analysis.

Supplier: STEMCELL Technologies

Chimeric mouse (V), human (C) monoclonal IgG antibody against SARS-CoV, SARS-CoV-2 (2019-nCoV) S protein (HEK293-expressed recombinant).

Supplier: STEMCELL Technologies

Medium for thawing and culturing hPSC-derived cardiomyocytes.

Supplier: STEMCELL Technologies

Fibroblast growth factor acidic (FGF-acidic), also known as FGF-1, is a potent activator of DNA synthesis, cell proliferation, and chemotaxis and is known to play numerous roles in development, regeneration, and angiogenesis (Jaye et al.; Galzie et al.; Presta et al.). FGF-acidic is produced by multiple cell types and is capable of activating all cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin. It is found in large quantities in the brain, but is also expressed in hepatocytes, vascular smooth muscle cells, neurons of the CNS, skeletal muscle cells, fibroblasts, keratinocytes, endothelial cells, intestinal columnar epithelial cells, and pituitary basophils and acidophils. FGF-acidic is secreted as a disulfide-linked homodimer and is stored in complex with heparan sulfate, a requirement for its interaction with FGF receptors (Guerrini et al.; Mohammadi et al.). Internalized FGF-acidic signals through protein kinase C and promotes cell survival by inhibiting p53 and proapoptotic signaling (Bouleau et al.).

Supplier: STEMCELL Technologies

Column-free immunomagnetic positive selection of human CD3+ and CD19+ cells (e.g. lymphocytes) cells for HLA analysis.

Supplier: STEMCELL Technologies

Immunomagnetic positive selection of mouse cells labelled with PE-conjugated antibodies.

Supplier: STEMCELL Technologies

Immunomagnetic isolation of untouched human leukocytes from whole blood.

Supplier: STEMCELL Technologies

Indian hedgehog (IHH) is one of three proteins in the hedgehog family which includes sonic hedgehog and desert hedgehog. Hedgehog proteins are important signaling molecules, acting through patched and smoothened receptors. During embryonic development, they are expressed in the fetal lung, gut, stomach, liver, kidney, and pancreas. IHH is strongly expressed in cartilage and regions of developing bone, and its signaling regulates growth and differentiation of bone tissue (Marigo et al.; Valentini et al.; St-Jacques et al.). IHH is also involved in yolk sac vasculogenesis, indicating a role in the differentiation of epiblast cells into endothelial and red blood cells (Byrd et al.). IHH induces the expression of parathyroid hormone-related peptide (PTHrP), which negatively regulates IHH during chondrocyte differentiation and proliferation (Vortkamp et al.).

Supplier: STEMCELL Technologies

Immunomagnetic depletion of human CD25+ cells.

Supplier: STEMCELL Technologies

Tumor necrosis factor-beta (TNF-β), also known as lymphotoxin-alpha, is a member of the TNF family. It is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways upon binding to TNF receptors 1 and 2. It is produced mainly by T cells, though other cells can express TNF-β at lower levels (Chu). TNF-β is involved in autoimmune disorders, lymph node development, and mediating the inflammatory demyelination process (Seleznik et al.; McCarthy et al.).

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Immunomagnetic isolation of eosinophils directly from whole blood

Supplier: STEMCELL Technologies

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing.

Supplier: STEMCELL Technologies

Granulocyte-macrophage colony-stimulating factor (GM-CSF) promotes the proliferation and differentiation of hematopoietic progenitor cells and the generation of neutrophils, eosinophils, and macrophages. In synergy with other cytokines such as stem cell factor, IL-3, erythropoietin, and thrombopoietin, it also stimulates erythroid and megakaryocyte progenitors (Barreda et al.). GM-CSF is produced by multiple cell types, including stromal cells, Paneth cells, macrophages, dendritic cells (DCs), endothelial cells, smooth muscle cells, fibroblasts, chondrocytes, and Th1 and Th17 T cells (Francisco-Cruz et al.). The receptor for GM-CSF (GM-CSFR) is composed of two subunits: the cytokine-specific α subunit (GMRα; CD116) and the common subunit βc (CD131) shared with IL-3 and IL-5 receptors (Broughton et al.). GM-CSFR is expressed on hematopoietic cells, including progenitor cells and immune cells, as well as non-hematopoietic cells. Recombinant human GM-CSF (rhGM-CSF) promotes the production of myeloid cells of the granulocytic (neutrophils, eosinophils and basophils) and monocytic lineages in vivo. It has been tested for mobilisation of hematopoietic progenitor cells and for treating chemotherapy-induced neutropenia in patients. GM-CSF is able to stimulate the development of DCs that ingest, process, and present antigens to the immune system (Francisco-Cruz et al.). This product is animal component-free.

Supplier: STEMCELL Technologies

Interleukin 6 (IL-6) is a pleiotropic growth factor with the wide range of biological activities in immune regulation, hematopoiesis, and oncogenesis. IL-6 is produced by a variety of cell types including T cells, B cells, monocytes and macrophages, fibroblasts, hepatocytes, vascular endothelial cells, and various tumor cell lines. On its own or in combination with other factors such as IL-2 and interferon-γ, IL-6 stimulates the proliferation of B cells, T cells, and hybridoma cells (Hirano et al.; Mihara et al.; Tanaka et al). In combination with cytokines such as IL-3, GM-CSF and SCF, IL-6 has been shown to promote hematopoietic progenitor cell proliferation and differentiation in vitro. IL-6 signals through a cell surface type I cytokine receptor complex consisting of the ligand-binding IL-6α (CD126) and the signal-transducing gp130 subunits. The binding of IL-6 to its receptor system includes activation of JAK/STAT signaling pathway (Mihara et al.; Peters et al.; Tanaka et al.). This product is animal component-free.

Supplier: STEMCELL Technologies

Glial cell line-derived neurotrophic factor (GDNF) is a neurotrophic factor and a member of the tumor growth factor (TGF)-beta superfamily. The GDNF family of growth factors also includes neurturin, persephin, and artemin, which have seven conserved cysteine residues called cysteine-knot (Treanor et al.). GDNF family ligands signal through binding to specific GDNF-family receptor-α (GFRα) co-receptors and activate the RET receptor tyrosine kinase (Durbec et al.). Four different forms of GFRα co-receptors have been characterized (GFRα 1-4) out of which GDNF binds specifically to GFRα1 prior to forming a complex with RET (Airaksinen and Saarma). GDNF is known to promote survival and morphological differentiation of midbrain dopaminergic neurons in both in vivo and in vitro studies and increase their high-affinity dopamine uptake (Granholm et al.; Lin et al.). GDNF has also been shown to have restorative effects on dying dopaminergic neurons in response to degenerative toxins (Aoi et al.). GDNF, together with Human Recombinant BDNF (brain-derived neurotrophic factor; Catalog #78005), BrainPhys™ Neuronal Medium (Catalog #05790), and other supplements, can be used to differentiate human pluripotent stem cell (hPSC)-derived neural progenitor cells into neurons (Bardy et al.). This product is animal component-free.

Supplier: STEMCELL Technologies

Heregulin-beta 1 also known as neuregulin-1 (NRG-1) is a member of the epidermal growth factor (EGF) family of growth factors and acts as a ligand for ErbB family receptor tyrosine kinases (Britsch et al.). Heregulin/neuregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3, and NRG4). Heregulin-beta 1 plays an important role during the development of the nervous system, heart, and mammary glands (Britsch). Heregulin-beta 1 is expressed in neuronal cells, and modulates cell growth and differentiation of the cells during development and wound healing (Mei and Xiong). It has been implicated through in vivo and in vitro studies that heregulin-beta 1/ErbB signaling is crucial for multiple aspects of cardiovascular development and protects the heart from ischemic injury (Odiete et al.). Heregulin-beta 1 also promotes invasiveness and metastasis of breast cancer cells (Hutcheson et al.). It has also been shown that heregulin-beta 1 has a role in the growth and maintenance of human embryonic stem cells (Wang et al.).

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Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220), unconjugated.

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For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing.

Supplier: STEMCELL Technologies

Rat monoclonal IgG2a antibody against human, mouse, cat CD45R (B220), biotin-conjugated.

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Mouse monoclonal IgG1 antibody against human, chimpanzee CD45, Pacific Blue™-conjugated.

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Immunomagnetic positive selection of mouse cells labeled with biotinylated antibodies.

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Immunomagnetic isolation of untouched human CD8+ T cells.

Supplier: STEMCELL Technologies

The platelet-derived growth factor (PDGF) family has five heparin-binding members that assemble into four homodimers (PDGF-AA, PDGF-BB, PDGF-CC, and PDGF-DD) and one heterodimer (PDGF-AB; Fretto et al.; Li and Eriksson). PDGF signals through the receptor tyrosine kinases PDGFRα and PDGFRβ. It has been shown that PDGF-induced migration involves signaling pathways involving MEK/ERK, EGFR, Src, and PI3K/AKT (Kim et al.). PDGF is a potent mitogen for cells of mesenchymal origin, such as fibroblasts, glial cells, and vascular smooth muscle cells. PDGF has been implicated in pathogenesis of atherosclerosis, glomerulonephritis, cancer, and in the contraction of vascular smooth muscle cells of rat aortic tissues (Fretto et al.; Sachinidis et al.). PDGF-BB is secreted by osteoblasts to induce mesenchymal stem cell migration and angiogenesis. It has also been shown that PDGF-BB is secreted by preosteoclasts during bone modeling and remodeling to induce angiogenesis and thus proper osteogenesis (Xie et al.).

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Fetal bovine serum for cell culture and research applications.

Supplier: STEMCELL Technologies

Hamster (Armenian) monoclonal IgG antibody against mouse CD11c, Pacific Blue™-conjugated.

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Kit for the detection of extracellular vesicles using CD9, CD63, and CD81 markers.

Supplier: STEMCELL Technologies

Rat (WI) monoclonal IgG2b antibody against mouse TER119.