903 Results for: "SIGMA ALDRICH MICROSCOPY"

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Brilliant cresyl blue is a phenoxazine dye.

Supplier: SIGMA ALDRICH MICROSCOPY

1,4-Dimethylpyridinium p-toluenesulfonate,98%

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Methylene blue, Neisser formulation. Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.

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Films are stained for Gram-positive organisms with crystal violet, mordanted in iodine, decolorized and then stained for Gram-negative organisms with safranin O. For delineating Gram-positive and Gram-negative organisms in films.

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2,7,12,17-Tetra-tert-butyl-5,10,15,20-tetraaza-21H,23H-porphine has been used in multifunctional drug delivery investigations from ionic triblock terpolymer micelles.

Supplier: SIGMA ALDRICH MICROSCOPY

Fast green FCF, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

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Neutral red (C.I. 50040) is a protonated diaminophenazine dye. It is aminodimethylaminomethylphenazinehydrochloride, C15H17N4Cl.

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Osmium tetroxide (OsO₄) is a heavy tetrahedral, symmetrical molecule composed of osmium, a third-row transition metal. Osmium tetroxide is both a dye, and a non-coagulant fixative agent in electron microscopy primarily used for the examination of biological tissue components in the histopathology laboratory. Specimens are postfixed with osmium testroxide to ensure preservation of the lipids.

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It is used for the preparation of electrically conducting glasses. Used to make solutions for the separation of RNA from DNA by density gradient centrifugation.

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4-(2-Pyridylazo)resorcinol (PAR) is a monosodium salt(1), dibasic acid that forms the protonated complexes with most metal ions.

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Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250. Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).

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3,4,5-Trihydroxybenzamide 98%, Sigma-Aldrich®

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7-Amino-4-(trifluoromethyl)coumarin (AFC) is a novel fluorescent marker used for the detection of proteinases. Suitable as laser dye.

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4'-Hydroxybiphenyl-4-carboxylic acid 99%, Sigma-Aldrich®

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Crystal violet, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Phycobiliprotein exhibiting exceptional absorbance and fluorescence characteristics due to a lack of susceptibility to internal and external fluorescence quenching. Ideal for highly sensitive studies in flow cytometry and immunoassays.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: SIGMA ALDRICH MICROSCOPY

Sudan IV has been used to in en-face staining of aorta, stains the lipid rich plaques. It has also been used to stain lipids atherosclerotic lesions of mice.

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3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

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Phenosafranin has been used for nuclear staining.

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DNA, RNA stain with advantageous spectral properties for cytometric applications.

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5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

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Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

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1-Aminoanthraquinone has the ability to increase the solubility of derivatives of anthraquinone in supercritical carbon dioxide.

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4-(2-(6-(Dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)pyridinium hydroxide inner salt has been used as a voltage sensitive dye in cardiac cells.

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3,4,5,6-Tetrabromophenolsulfonephthalein, Dye content 95 %

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The Epoxy Embedding Medium Kit is a very widely used embedding medium for electron microscopy. The embedding formulation originally published by Luft (1961) is excellent for both plant and animal tissues. Due to the low viscosity of the resin, it penetrates the tissue specimen faster than Araldite and other polymers. It hardens easily and uniformly at low temperatures with the addition of dodecenylsuccinic anhydride (DDSA), methyl nadic anhydride (MNA), and the accelerator 2,4,6-tris(dimethylaminomethyl)phenol (DMP-30). Slight shrinkage may occur during curing. This kit is useful for embedding a variety of tissues as a wide range of hardness can be obtained with this resin to suit a specific tissue type by using two different anhydride curing agents (DDSA and MNA).

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Tetramethylrhodamine methyl ester perchlorate (TMRM) is a fluorescent dye that can label mitochondria and mark embryonic stem cell/induced pluripotent stem cell-derived cardiomyocytes. It is useful for the isolation of mature cardiomyocytes.

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May-Grünwald stain is a Romanowsky neutraldye stain based on the combination ofmethylene blue azures as the basic dyecomponent and eosin as the acid dyecomponent.It is intended for use in staining blood films orbone marrow films. It is used routinely for diagnostic purposes in hematology.