74276 Results for: "MP Biomedicals"

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Poly-lysine is a polycation which binds to DNA, red cell membrane and any negatively charged protein. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.
It is typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. Both the D- and L- form of the poly-lysine can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. In this case, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine.
Other uses for poly-lysine have been reported as well:
• Conjugation to methotrexate for increased drug transport.
• Microencapsulation of islets.
• Use in simple reproducible procedure for chromosomal preparations from a variety of tissues.
• Immobilation of living cells in biocompatible semipermeable microcapsules.
• In the preparation of polycationic beads.
• Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Poly-D-lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has additionally been shown to eliminate prion proteins from infected cells.

Supplier: MP Biomedicals

SPINeasy DNA Kit for Water is a high-performance water genomic DNA (gDNA) extraction kit based on silica-membrane spin-column technology. This kit enables quick isolation of gDNA from water in less than 30 min. Water samples are processed using our uniquely formulated Lysis Buffer W1 and Lysing Matrix E to effectively lyse various types of cells. Column W1 provided in the kit has high binding capacity and selectivity for gDNA. The combination of components in the kit extracts gDNA of high yield and purity that is ready for downstream analyses such as PCR, restriction digestion and sequencing.

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2-Aminoanthracene

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Fluorescein diacetate

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Alizarin red S is used in a biochemical assay to determine, quantitatively by colorimetry, the presence of calcific deposition by cells of an osteogenic lineage.

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Iodoacetamide is a thiol reagent; alkylating reagent for cysteine and histidine residues in proteins. In the alkylation reaction, it reacts with histidine (such as in RNase), methionine and sulfhydryl groups of many proteins.

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Lithium cobaltite (lithium cobalt(III) oxide)

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Synthetic Bactericidal Antibiotic. A structural analog of D-Alanine. A partial agonist at the glycine modulatory site of NMDA receptors; enhances learning and memory in several models of cognitive deficit; anticonvulsant (at high doses). Antibiotic against gram-negative bacteria that acts by inhibiting the synthesis of the cell wall (D-Ala peptide bond formation).

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Hemoglobin is the major oxygen-transporting component of erythrocytes. Isolated from bovine blood.

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L-α-Glycerophosphoryl Choline, Cadmium Chloride Complex (from egg yolk) ∼98%, white powder

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Soluble in water (100 mg/ml, swells and dissolves slowly); insoluble in alcohol.

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Rotenone is a naturally occurring botanical compound obtained from the roots of Derris sp.& Cube. It is an inhibitor of mitochondrial electron transport, specifically inhibiting NAD-linked substrate oxidation by mitochondria at the oxygen side of NADH dehydrogenase.
Rotenone is utilized as an insecticide. It can be used as neurotoxic agent that can produce a Parkinson-like condition as an animal model for study of etiology and interventions.
Inhibitor of mitochondrial electron transport at NADH: ubiquinone oxidoreductase. It is readily absorbed through the exoskeletons of arthropods, but poorly absorbed cutaneously or from the gastrointestinal tract of mammals. Rotenone is used to induce a Parkinson-like syndrome as an experimental model in rats.

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Adenosine 5'-monophosphate disodium salt (AMP disodium salt) hydrate ≥99%, powder

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Bismarck brown Y is a Plasma stain used for staining mucine, cartilage and goblet cells.

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Freely soluble in water.

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-Aminosalicylic acid is an analogue of p-aminobenzoic acid. It is an antitubercular agent. The sodium salt of the drug is better tolerated than the free acid.

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3,4-Dimethoxybenzoic acid

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The methylated xanthine compounds caffeine, theobromine, and theophylline and their derivatives are used in medicine for their bronchodilator effects.

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SPINeasy PCR purification and gel extraction Kit is a silica-membrane spin-column kit that enables quick and convenient DNA clean up from various enzymatic reactions, such as PCR and restriction digestion, as well as isolation and purification of DNA fragments from agarose gel electrophoresis.

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SPINeasy Plasmid Midiprep kit is a silica-membrane spin-column kit for the purification of high-copy and low-copy number plasmid DNA from 25 to 50 ml of transformed bacterial (E.coli) culture.

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Humic acids, heavy metals, heme are the most notorious PCR inhibitors. This could be due to non-optimised DNA purification procedures which often co-purify inhibitors and lead to false negative results on downstream applications.

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SPINeasy Virus RNA kit is a silica-membrane spin-column kit that enables quick and convenient extraction of virus RNA from cell culture media and bodily fluids such as saliva and serum, without the use of toxic substances such as phenol and chloroform. Through a simple workflow, virus RNA is typically extracted within 20 minutes and is immediately available for downstream applications such as RT-PCR and RT-qPCR.

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Guanidine Hydrochloride is a strong chaotropic agent useful for the denaturation and subsequent refolding of proteins.

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Glucose-6-phosphate dehydrogenase (G6PD or G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH).

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Gallic acid monohydrate, powder ACS

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Conduritol B Epoxide (CBE) is a potent, irreversible inhibitor of various plant β-glucosidases and mammalian glucocerebrosidase, specific covalent inhibitor of β-glucosidase which results in the accumulation of glucocerebroside.

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Protein kinase C inhibitor (IC50 = 50-200 µM depending on assay conditions in MCF-7 cells or IC50 = 100 µM in rat brain). The PKC inhibition is also dependent on the phosopholipid concentration. Also inhibits both calmodulin-dependent and calmodulin-independent Ca2+-, Mg2+-ATPase. Tamoxifen is a selective estrogen response modifier (SERM), anti-angiogenetic factor. It is a prodrug that is metabolized to active metabolites 4-hydroxytamoxifen (4-OHT) and endoxifen by cytochrome P450 isoforms CYP2D6 and CYP3A4. In breast cancer, the gene repressor activity of tamoxifen against ERBB2 is dependent upon PAX2. Blocks estradiol-stimulated VEGF production in breast tumor cells.
Tamoxifen has been used to facilitate the recombination of ect2flox allele in mouse organs. It has also been used to study its effect on lipopolysaccharide (LPS)-induced microglial activation
Tamoxifen is a Protein kinase C inhibitor. It induces apoptosis in human malignant glioma cell lines. Tamoxifen and its metabolite 4-hydroxytamoxifen are selective estrogen response modifiers (SERMs) that act as estrogen antagonists in mammary gland. Blocks estradiol-stimulated VEGF production in breast tumor cells.

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Sulfobromophthalein (BSP) is a dye with a high affinity for organic anion transporting polypeptides (OATPs) and has been used as a substrate for multidrug resistance associated protein 2 (Mrp2). BSP is transported into hepatocytes by OATPs and, after conjugation to glutathione, is excreted into bile by Mrp2. It was found to inhibit the aldo-keto reductase ARK1C20.
Sulfobromophthalein (BSP) is used in diagnosis of hepatic disorders. It is also used for the quantitative determination of proteins.

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Rapamycin binds to and inhibits the molecular target of rapamycin (mTOR). It forms a complex with FKBP12 that binds to and inhibits the molecular target of rapamycin (mTOR). Rapamycin is a potent immunosuppressant and has anticancer activity.

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Hypotaurine is a Non-specific, endogenous inhibitory glycine receptor agonist.