74275 Results for: "MP Biomedicals"

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Butylated hydroxytoluene is a phenolic antioxidant.

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Polymyxin B sulphate is a mixture of Polymyxin B1 and Polymyxin B2 sulphate, obtained from Bacillus polymyxia strains. Polymyxin B inhibits gram-negative organisms in vitro wherein it binds to and interferes with the permeability of the bacterial cytoplasmic membrane and is also reported to inhibit Ca²⁺ dependent protein kinase C (PKC). Effective concentration is typically 50 mg/l.

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Lymphocyte Separation Medium (LSM™) is formulated for isolation of mononuclear cells from defibrinated or heparinised whole human blood. One-step centrifugation permits separation of mononuclear lymphocytes from erythrocytes, polynuclear lymphocytes and most platelets. It is a sterile-filtered solution of a sucrose polymer and diatrizoate salts.

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Each impact-resistant 2 ml tube contains 0,8 mm zirconium silicate beads.

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L-Methionine

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Preparation of cDNA templates for qPCR.

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Soluble in water, EGME, ethanol.

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IBMX has been shown to be a potent, non-specific inhibitor of adenosine 3',5'-cyclic monophosphate phosphodiesterase (cAMP PDE)4, significantly more effective than theophylline. Also inhibits cGMP phosphodiesterases. IBMX inhibits cyclic nucleotide PDE with subsequent inhibition of cyclic nucleotide hydrolysis, resulting in accumulation of cyclic AMP and guanosine 3',5'-cyclic monophosphate.

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Rifamycin SV is a broad-spectrum semisynthetic antimicrobial agent of the rifamycin group.

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Suitable for both protein and nucleic acid isolation. Exhibits proteolytic activity on proteins, peptides, glycoproteins, amides and esters. Also active with nitroanilides of amino acids with protected amino groups, excluding arginine. Useful in the isolation of DNA and RNA, in the analysis of membrane structures and protein structure. Proteolytic activity: >30mAnson Units/mg Unit definition: One Anson unit is the amount of enzyme which liberates 1 µmol of Folin-positive amino acids per minute at pH 7,5 and 35 °C, using hemoglobin as substrate.

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Mounting medium for mycloplasma HOECHST stain kit

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Each impact-resistant 2 ml tube contains 1 mm silica spheres. Lysing Matrix C tubes have red caps and are found in the FastRNA™ Pro Red Kit and FastProtein Red Matrix. Lysing Matrix C is used primarily for lysis of yeast and fungi and can also be used for moulds, spores and filamentous algae.

Supplier: MP Biomedicals

Each impact-resistant 2 ml tube contains garnet matrix and one ¼ inch ceramic sphere. Extra ¼" ceramic spheres are packaged separately. Lysing Matrix A tubes have orange caps and are found in the FastDNA™ and FastDNA™ SPIN Kits. Lysing Matrix A is used for all sample types except soil for the subsequent isolation of genomic DNA, RNA, functional proteins and other metabolites and small molecules. This grinding matrix is chemically inert and will not bind nucleic acids.

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Vinblastine Sulphate is an anti-tumor alkaloid.

Supplier: MP Biomedicals

Storage: -20°C, desiccate
This is an ultrapure NAD, chromatographically purified to remove trace inhibitors.
β-NAD, a pyridine nucleotide and biologically active form of nicotinic acid, is a coenzyme necessary for the catalytic reaction of certain enzymes. It occurs in living cells primarily in the oxidized state. Serves as a coenzyme of the dehydrogenases, especially in the dehydrogenation of primary and secondary alcohols. NAD usually acts as a hydrogen acceptor, forming NADH which then serves as a hydrogen donor in the respiratory chain.
Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.

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These antibodies specifically reacts with human IgG1 (Fc region).

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Polyethylenimine is a branched chain polymer having a ratio of 1:2:1 of primary:secondary:tertiary amines with a branching site every 3 to 3,5 nitrogen atoms and a base structure of (CH₂CH₂NH)x. The nitrogen atoms must be protonated to achieve cationic charge, so the cationic charge density is pH dependent.

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Human native IL-6

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Superoxide dismutases are enzymes that catalyse the dismutation of superoxide into oxygen and hydrogen peroxide. They are an important antioxidant defense in nearly all cells exposed to oxygen.

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Eosin Y disodium salt is used in combination with Azure II to show morphological details of marrow cells; buffered Azure A/Eosin Y method for demostrating cell granules, microorganisms and nuclei; for showing insect musculature; for demonstrating cells infected with mosquito-iridescent virus; use in combination with Alum Hematoxylin/Azure II to show mast cells, early necrobiotic changes in cells and protozoans, rickettsias and bacteria in tissues; to demonstrate volutin granules in any microorganism; Bond's modification of Mann's Eosin-Methyl Blue for Negri bodies in smears; Harris stain for Negri bodies in smears; Mann's method for staining Negri bodies in sections; Gridley's stain for Endamoeba in tissue sections; a modified methenamine-silver nitrate method for the demonstration of Endamoeba in sections. It is used as a counterstain for hematoxylin.

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The hyaluronidase is purified from Streptomyces hyalurolyticus. This enzyme belongs to the endo-beta-hexosaminidase and endo-eliminase type enzyme that produces oligo-saccharide (unsaturated tetraose and pentose) by hydrolysing the beta-1,4-linkage of hyaluronic acid. It is stable in both high temperature and acid or alkaline range. It has high substrate specificity and hydrolyzes hyaluronic acid only in mucopolysaccharide.

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Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis.

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MPure™ Viral/Pathogen Nucleic Acids Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for purification of viral DNA/RNA and pathogen DNA from swab samples (cell-rich samples).

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MPure™ Forensic DNA Extraction Kit is used with the MPure-12™ aNAP system for the extraction and purification of genomic DNA from forensic samples.

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MPure™ Tissue DNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction and purification of genomic DNA from a variety of animal tissues, swab samples and blood stains. Nucleic acids extracted and purified from MPure™ Tissue DNA Extraction Kits can be used in a number of downstream applications including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP and Southern Blot Analysis.

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Goat IgG fraction to beta-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques.
The antibody is produced by hyperimmunizing goats with the enzyme beta-galactosidase from Escherichia coli. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-beta-galactosidase E. coli proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.

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Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.It is also used in indirect ELISA, immunofluorescence, immunoblots and immunohistochemistry

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Tannic Acid is used for enzyme immobilization and protein adsorption

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o-Nitrophenyl-β-D-galactoside is a cell culture component for molecular genetics. It is chromogenic substrate for b-galactosidase and hence is useful in the detection of lacZ activity.

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Oleic acid is a mono-unsaturated omega-9 fatty acid created by dehydrogenation of stearic acid. It is then further dehydrogenated and elongated to create members of the n-9 family of fatty acids.