Mouse IL-1ra ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse IL-1ra in serum, plasma, and other biological fluids. This ELISA kit only requires 25 µl of sample volume(s).

• Small Sample Volume: Requires as little as 25 µl of sample!
• Assay Precision: Intra-Assay: CV <10%, Inter-Assay: CV <10%

Mouse IL-1ra ELISA kit employs the sandwich Enzyme-Linked Immunoassay technique for the quantitative measurement of mouse IL-1ra in serum, plasma, and other biological fluids. An antibody specific for IL-1ra has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IL-1ra present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IL-1ra Antibody, which binds the captured IL-1ra present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Avidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IL-1ra captured in each well. The concentration of IL-1ra can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.