- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Rabbit
- Target protein:TNF beta
- Description:Rabbit TNF beta ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:Rabbit TNF beta ELISA Kit exhibits high specificity and excellent specificity for the detection of rabbit TNF beta. No significant cross-reactivity or interference between TNF beta and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:15,625 - 1000 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:9,375 pg/ml
- Regulatory status:RUO
- Tests per kit:96 tests
Rabbit TNF beta ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rabbit TNF beta in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Rabbit TNF beta ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rabbit TNF beta in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for TNF beta has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the TNF beta present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-TNF beta Antibody, which binds the captured TNF beta present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of TNF beta captured in each well. The concentration of TNF beta can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.