EasySep™ Release Human CD4 Positive Selection Kits

Supplier: STEMCELL Technologies

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Supplier: STEMCELL Technologies

About this item

Immunomagnetic positive selection of particle-free human CD4+ cells.

Highly purified human CD4+ cells isolated in less than 30 minutes
No-wash removal of EasySep™ Releasable RapidSpheres™

The EasySep™ Release Human CD4 Positive Selection Kit is designed to isolate CD4+ cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD4+ cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands. The CD4 antigen is strongly expressed on helper T cells and is expressed more weakly on peripheral blood monocytes and tissue macrophages.

Caution: Product Restrictions: (i) Customer shall not make products or any portion of them, in any way, shape or form, including as a component of another product available for the purpose of further resale or alter or remove the Product label and the Supplier’s mark of origin without express written permission of Supplier; and (ii) Customer is familiar with Supplier’s restrictions on the use of the Products and shall use the Products in compliance with such restrictions and the applicable Supplier Product Information sheet (collectively, the ‘Product Restrictions’).