- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:LOXL2
- Description:Human LOXL2 ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human LOXL2 ELISA kit exhibits high specificity and excellent specificity for the detection of human LOXL2. No significant cross-reactivity or interference between LOXL2 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:37,5 - 2400 pg/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:22,5 pg/ml
- Regulatory status:RUO
Human LOXL2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human LOXL2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Detection range: 37,5 to 2400 pg/ml
- Sensitivity: 22,5 pg/ml
- Sample type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Human LOXL2 employs the sandwich enzyme immunoassay technique for the quantitative measurement of human LOXL2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for LOXL2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the LOXL2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-LOXL2 Antibody, which binds the captured LOXL2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of LOXL2 captured in each well. The concentration of LOXL2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.