DH5α Chemically Competent E. coli Cells
DH5α chemically competent cells (≥10⁸ CFU/μg of pUC19 DNA), Chemically competent accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
Catalog # ORIGCC100003
Supplier: OriGene
Jump to:
Product Details & Documents
- lacZ marker for blue/white colour screening
- Transformation of cloned DNA into bacterial cells
- Ideal for subcloning and generating cDNA libraries
Kit contains:
DH5α chemically competent cells (5×200 μl)
Positive control DNA - pUC19 (50 pg/μl, 25 μl)
SOC medium: 20 ml
Specifications
- Description:DH5α chemically competent cells (≥10⁸ CFU/μg of pUC19 DNA)
- Feature:Chemically competent accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
- Cat. No.:ORIGCC100003
- Storage conditions:The competent cells should be stored at −80 °C, pUC19 control DNA should be stored at −20 °C and SOC recovery medium should be stored at 4 °C immediately upon arrival
- Genotypes:F – φ80lacZDM15 D(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk–, mk+) phoA supE44 l – thi–1 gyrA96 relA1
Empfehlungen
Empfehlungen werden nur dann basierend auf Ihren Einkaufspräferenzen personalisiert, wenn Sie auf der Seite "Persönliche Daten" die Option „Mein Einkaufserlebnis verbessern“ aktiviert haben.
Andernfalls erhalten Sie allgemeine Empfehlungen.
Acetonitrile, anhydrous (max. 0.003% H₂O) ≥99.95%, HiPerSolv CHROMANORM® Reag. USP, Reag. Ph. Eur., ACS, super gradient grade suitable for UPLC/UHPLC instruments
Katalog #: 83639.320
Supplier: VWR Chemicals
Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC
Katalog #: 20864.320
Supplier: VWR Chemicals
Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments
Katalog #: 83640.320
Supplier: VWR Chemicals
Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®
Katalog #: 1.00030.2500
Supplier: Merck
Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution
Katalog #: 31770.294
Supplier: VWR Chemicals
Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments
Katalog #: 83645.320
Supplier: VWR Chemicals
Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®
Katalog #: 1.09439.1000
Supplier: Merck