Culture medium kit for differentiation of human gastric organoids.

Reliably generate hPSC-derived gastric organoids for studying gastric development, inflammation, regeneration, microbial interaction, or for disease modeling. With the STEMdiff™ Gastric Organoid Differentiation Kit, you can grow and expand organoids across hPSC lines with high efficiency and reproducibility, to form a convenient model system with direct relevance to the developing stomach. This kit’s simple, four-stage protocol, based on publications by McCracken et al. (Nature 2014) and Bartfeld et al. (Gastroenterology 2015), enables the generation and differentiation of gastric organoids directly from hPSCs as well as the long-term expansion and cryopreservation of gastric organoids. Organoids cultured with this kit exhibit a cellular composition and organisation that recapitulates that of the developing gastric epithelium and associated mesenchyme, providing a physiologically relevant model system for gastric research, as well as drug discovery and compound screening. This kit is optimised for differentiation of cells maintained in mTeSR™1 or mTeSR™ Plus.