Kit including BrainPhys™ Neuronal Medium, SM1 Neuronal Supplement, N2 Supplement-A, BDNF and GDNF for culture of ES/iPS cell-derived neurons.

Culture, differentiate, and mature neurons derived from human embryonic stem (ES) or induced pluripotent stem (iPS) cells by using a complete medium optimised to promote, rather than inhibit neuronal activity. For your convenience, BrainPhys™ hPSC Neuron Kit includes serum-free BrainPhys™ Neuronal Medium (basal medium), supplements, and growth factors to enable generation and maturation of different neuronal subtypes from human ES/iPS cell-derived neural progenitor cells. Based on the formulation, BrainPhys™ Neuronal Medium mimics the extracellular environment of the central nervous system (CNS) to yield a higher proportion of synaptically active neurons.

NeuroCult™ SM1 Neuronal Supplement ensures cell health and encourages neurite outgrowth and branching in short- and long-term serum-free cultures, and N2 Supplement-A supports the in vitro differentiation of ES/iPS-derived cells to neuronal subtypes. Included BDNF and GDNF growth factors support lineage-specific differentiation. To avoid shocking cells with media changes, also use BrainPhys™ medium when performing functional assays, such as microelectrode array-based recordings or live-fluorescent imaging. View our additional resources to learn more about the BrainPhys™ system.