Semi-solid methylcellulose-based medium for selection and cloning of suspension-adapted and adherent cells (serum-containing).

• Individual cells are suspended in viscous medium and form physically separated, discrete colonies that are easily isolated
• Monoclonal cell lines are isolated in less time using fewer resources compared with selection and cloning by limiting dilution
• Diverse clones with a wide range of growth rates and productivities form discrete colonies in the viscous medium
• Rare and high-producing clones can be individually isolated more easily using simultaneous selection and cloning in semi-solid medium compared with selection in bulk liquid cultures

ClonaCell™-TCS Medium is a serum-containing methylcellulose-based semi-solid medium that can be used for selecting and cloning a variety of suspension-adapted cell lines, including CHO-S and hybridomas. It can also be used for semi-solid cloning of some cell lines that grow adherently in the presence of serum, including CHO-K1, BHK-1, and HEK-293. This medium contains pre-selected fetal bovine serum (FBS) and bovine serum albumin (BSA) and supports robust growth of a wide variety of cell types. ClonaCell™-TCS Medium does not contain selection agents.